Identification of essential survival genes

Details Identification of essential survival genes Identification of essential survival genes

1996-05-06

1998-05-26

Degen, Nancy

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 29, 4351721, 4352521, 4352528, 4352534, 4352541, 4352551, C12Q 104, C12N 114, C12N 120, C12N 1501

Patent

active

057563055

ABSTRACT:
A method for identifying a strain having a conditional lethal mutation which is essential for survival when strain is incubated under restrictive growth conditions, and methods of identifying gene products and gene functions thereof.

REFERENCES:
Lodge et al., "Comparison of Myristoyl-CoA:Protein N-Myristoyltransferases from Three Pathogenic Fungi: Cryptococcus neoformans, Histoplasma capsulatum, and Candida albicans," J. Biol. Chem, 28:2996-3009, 1994.
Schmid et al., "Genetic Analysis of Temperature-Sensitive Lethal Mutants of Salmonella typhimurium," Genetics, 123:625-633, 1989.
Smith et al., "Hyphal Tip Extension in Aspergillus nidulans Requires the manA Gene Which Encodes Phosphomannose Isomerase," Mol. and Cell. Biol., 14:6030-6038, 1994.
van Zyl et al., "A General Screen for Mutants of Saccharomyces cerevisiae Deficient in tRNA Biosynthesis," Genetics, 123:55-68, 1989.
Winston, "Mutagenesis of Yeast Cells," Current Protocols in Molecular Biology, Supplement 19, 2:13.3.1-13.3.4, 1992.
Inoko et al. "Isolation & Characterization of Conditional-Lethal Rho Mutants of Eschericia coli " DNAS 74(3) 1162-1166, Mar. 1977.
Hou et al. "In Vivo Selection of Conditional-Lethal Mutations in the Gene Ecoding Elongation Factor F of Escherichia coli" J Bacteriol. 176(1) 123-129, Jan. 1994.
Bender et al., "Use of a Screen for Synthetic Lethal and Multicopy Suppressee Mutants to Identify Two New Genes Involved in Morphogenesis in Saccharomyces cerevisiae," Mol. and Cell. Biol., 11:1295-1305, 1991.
Costigan et al., "NHP6A and NHP6B, Which Encode HMG-1-Like Proteins, Are Candidates for Downstream Components of the Yeast SLT2 Mitogen-Activated Protein Kinase Pathway," Mol. and Cell. Biol. 14:2391-2403, 1994.
Denison et al., "Mutation in the bimD Gene of Aspergillus nidulans Confers a Conditional Mitotic Block and Sensitivity to DNA Damaging Agents," Genetics, 134:1085-1096, 1993.
Gietz et al., "Improved method for high efficiency transformation of intact yeast cells," Nucleic Acids Research, 20(6):1425, 1992.
Harris et al., Molecular Analysis of Saccharomyces cerevisiae Chromosome I On the Number of Genes and the Identification of Essential Genes Using Temperature-sensitive-lethal Mutations, J. Mol. Biol., 225:53-65 1992
Hooke, "Temperature-Sensitive Mutants of Bacterial Pathogens: Isolation and Use to Determine Host Clearance and in Vivo Replication Rates," Methods in Enzymology, 235:4458-457, 1994.
Karrow et al., "The essential Escherichia coli msbA gene, a multicopy suppressor of null mutations in the htrB gene, is related to the universally conserved family of ATP-dependent translocators," Mol. Microbiol., 7(1):69-79, 1993.
Leidich et al., "Temperature-sensitive Yeast GPI Anchoring Mutants gpi2 and gpi3 Are Defective in the Synthesis of -Acetylglucosaminyl Phosphatidylinositol," J. Biol. Chem., 270:13029-13035, 1995.

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