Identification of differentially methylated and mutated nucleic

Details Identification of differentially methylated and mutated nucleic Identification of differentially methylated and mutated nucleic

1996-05-31

1999-02-16

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 536 231, 536 243, 536 2433, C07H 2104, C07H 2103, C12Q 168, C12P 1934

Patent

active

058719172

ABSTRACT:
The present invention provides Methyl- (or Mutant-) Differential Display (MDD) methods and nucleic acid probes for detecting mutations and the methylation patterns of nucleic acids. The methods of the present invention are particularly useful for detecting and isolating genomic DNA fragments which are near coding and regulatory regions of genes and which are differentially mutated or methylated relative to the corresponding DNA from normal cells. Genes are frequently not methylated in the cells where they are expressed but are methylated in cell types where they are not expressed. Moreover, tumor cell DNA is frequently methylated to a different extent and in different regions than is the DNA of normal cells. The present invention is used for identifying which regions of the genome are methylated or mutated in different cell types, including cancerous cell types. The present invention is also used for diagnosing whether a tissue sample is cancerous, and whether that cancerous condition is non-metastatic or metastatic.

REFERENCES:
patent: 5436142 (1995-07-01), Wigler et al.
Clark et al, "CpNpGp Methylation in Mammalian Cells", Nature Genetics, vol. 10, May 1995, pp. 20-27.
Little et al, "Methylation and p16; Suppressing the Suppressor", Nature Medicine, vol. 1, No. 7, Jul. 1995, pp. 633-634.
Merlo et al, "5' CpG Island Methylation is Associated with Transcriptional Silencing of the Tumour Suppessor p16/CDKN2/MTS1 in Human Cancers", Nature Medicine, vol. 1 No. 7, Jul. 1995, pp. 686-692.
Parrish et al, "Methods for Finding Genes A Major Rate-Limiting Step in Positional Cloning", GATA, vol. 10, No. 2, 1993, pp. 29-41.
Lisitsyn et al, "Cloning the Differences Between Two Complex Genomes", Science, vol. 259, Feb. 12, 1993, pp. 946-951.
Laird et al, "DNA Methylation and Cancer", Human Molecular Genetics, vol. 3, 1994, pp. 1487-1495.
Xu et al, "New Polymorphic Markers in the Vicinity of the Pearl Locus on Mouse Chromosome 13",Mammalian Genome, vol. 7, 1996, pp. 16-19.
Wieland, et al, "A Method for Difference Cloning: Gene Amplification Following Subtractive Hybridization", Proc. Natl. Acad. Sci, USA 87, 1990, pp. 2720-272.
Braun et al, "Identification of Target Genes For The Ewing's Sarcoma EWS/LFI Fusion Protein by Representational Difference Analysis", Molecular and Cellular Biology, vol. 15, No. 8, Aug. 1995, 4623-4630.
Chang, et al, "Identification of Herpesvirus-Like DNA Sequences in AIDS-Associated Kaposi's Sarcoma", Science, vol. 266, Dec. 16, 1994, pp. 1865-1869.
Hubank et al, "Identifying Differences in mRNA Expression by Representational Difference Analysis of cDNA", Nucleic Acids Research, vol. 22, No. 26, 1994, pp. 5640-5648.
Lisitsyn et al, "Direct Isolation of Polymorphic Markers Linked to a Trait by Genetically Directed Representational Difference Analysis", Nature Genetics, vol 6, Jan. 1994, pp. 57-63.
Issa, et al, "The Estrogen Receptor CpG Island Is Methylated in Most Hematopoietic Neoplasms", Cancer Research, vol. 56, Mar. 1, 1996, pp. 973-977.
Costello et al, "Silencing of p16/CDKN2 Expression in Human Gliomas by Methylation and Chromatin Condensation", Cancer Research vol. 56, May 15, 1996, pp. 2405-2410.
Loebel et al, "Methylation Analysis of a Marsupial X-linked CpG Island by Disulfite Genomic Sequencing", Genome Research vol. 6, 1996 pp. 114-123.
Frommer et al, "A Genomic Sequencing Protocol that Yields a Positive Display of 5-Methylcytosine Residues in Individual DNA Strands", Genetics, vol. 89, Mar. 1992, pp. 1827-1831.
Horsthemke, et al, "PCR-Mediated Cloning of Hpall Tiny Fragments from Microdissected Human Chromosomes", PCR Methods and Applications, vol. 1, 1992, pp. 229-237.
McGrew et al, "Quantitation of Genomic Methylation Using Ligation Mediated PCR", BioTechniques, vol. 15, No. 4, 1993, pp. 722-724.
Singer-Sam et al, "A Quantitative Hpall-PCR Assay to Measure Methylation of DNA From a Small Number of Cells", Nucleic Acids Research, vol. 18, No. 3, Dec. 4, 1989, p. 687.
Steigerwald, et al, "Ligation-Mediated PCR Improves the Sensitivity of Methylation Analysis by Restriction Enzymes and Detection of Specific DNA Strand Breaks", Nucleic Acids Research, vol. 18, No. 6, Dec. 15, 1989, pp. 1435-1439.
Graff et al, "E-Cadherin Expression Is Silenced by DNA Hypermethylation in Human Breast and Prostate Carcinomas", Cancer Research, vol. 55, Nov. 15, 1995, pp. 5195-5199.
Belinsky, et al, "Increased Crytosine DNA-Methyltransferase Activity is Target-Cell-Specific and An Early Event in Lung Cancer", Genetics, vol. 93, Apr. 1996, pp. 4045-4050.
Willison, "Opposite Imprinting of the Mouse Igf2 and Igf2r Genes", Trends in Genetics vol. 7, 1991, pp. 107-109.
Clark et al. Nature Genetics 10: 20-27, 1995.
Fieler and Jacobs Plant Mol. Biol. 17: 321-333 (Attached Genbank Sequence Listing), 1991.

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