Identification of differentially expressed genes

Details Identification of differentially expressed genes Identification of differentially expressed genes

1995-06-07

1997-12-23

Chambers, Jasemine C.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 9151, 4351723, 4353201, 435915, 435 912, C12Q 168, C12P 1934, C12N 1510, C12N 1570

Patent

active

057006440

ABSTRACT:
Methods are disclosed to identify differentially expressed genes. In one aspect, one uses subtractive hybridization to enrich for candidate genes, followed by a PCR amplified radiolabeled display of cDNA products of the hybridization. Specially modified primer binding regions are used that can be achieved either through use of trimming plasmids or non-symmetric display plasmids.

REFERENCES:
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Hakvoort et al., "Identification of enriched sequences from a cDNA subtraction-hybridization procedure", Nucleic Acids Res. 22: 878-879, Mar. 1994.
Z. Wang, et al., A gene expression screen, 88 Proc. Natl. Acad. Sci. U.S.A. 11505-11509 (1991).
P. Liang et al., Differential Display of Eukaryotic Messenger RNA By Means OF The Polymerase Chain Reaction, 257 Science 967-971 (1992).
E. Arizai et al., Cloning Of cDNAs Differentially Expressed Between Regressing And Non-regressing Rat Mammary Carcinomas Induced By Monoterpenes, 35 Proc. Annu. Meet. Am. Assoc. Cancer Res. (Mar. 1994).
N. Hasan et al., An Mboll/Fokl Trimming Plasmid Allowing Consecutive Cycles Of Precise 1-To 12-Base-Pair Deletions In Cloned DNA, 82 Gene 305-311 (1989).
N. Hasan et al.,A Novel Multistep Method For Generating Precise Unidirectional Deletions Using Bspmi, A Class-IIS Restriction Enzyme, 50 Gene 55-62 (1986).

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