Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1993-02-19
1999-02-23
Scheiner, Laurie
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 5, 530350, 530300, G01N 3353, C12Q 170, C07K 100, C07K 1400
Patent
active
058742255
ABSTRACT:
The present invention is directed toward methods of identifying compounds which inhibit the human immunodeficiency virus (HIV) viral protein R (Vpr) from stimulating the differentiation of undifferentiated cells. This invention takes advantage of the observation that cell lines from rhabdomyosarcomas, which are tumors of muscle origin, have been used as models of CD4-independent HIV infection. These cell lines can be induced to differentiate in vitro. The vpr gene of HIV-1 is sufficient for the differentiation of the human rhabdomyosarcoma cell line TE671. Differentiated cells are characterized by great enlargement, altered morphology, lack of replication, and high level expression of the muscle-specific protein myosin. Morphological differentiation and inhibition of proliferation of other transformed cell lines following vpr expression was also observed. This invention also relates toward methods of identifying compounds which inhibit HIV Vpr binding to Gag. These screening methods should facilitate the identification and development of antiviral agents.
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Levy David Nathan
Weiner David B.
Parkin Jeffrey S.
Scheiner Laurie
The Wistar Institute
Trustees of the University of Pennsylvania
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