Identification of cell density signal molecule

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Hormones – e.g. – prolactin – thymosin – growth factors – etc.

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530356, 530414, 530427, 530840, C07K 114, C07K 134, C07K 14465, C07K 1450

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active

057418950

ABSTRACT:
Disclosed herein is a novel proteinaceous cell density signal molecule (CDS) between 25 and 35 kD, which is secreted by fibroblastic primary avian tendon cells in culture, and causes the cells to self-regulate their proliferation and the expression of differentiated function. It effects an increase of procollagen production in avian tendon cell cultures of ten fold while proliferation rates are decreased. CDS, and the antibodies which recognize them, are important for the development of diagnostics and treatments for injuries and diseases involving connective tissues, particularly tendon. Also disclosed are methods of production and use.

REFERENCES:
patent: 4731357 (1988-03-01), Franklin et al.
Harris et al. Protein Purification Methods. Oxford: IRL Press. 1989, pp. 9,10,57-60,62.
Martis et al, A Simple Fractionation of Chicken Egg Yolk . . . In Vitro Cellular & Developmental Biol. May 1986, vol. 22, No. 5, pp. 241-246.

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