Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Reexamination Certificate
2011-01-18
2011-01-18
Chunduru, Suryaprabha (Department: 1637)
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
C435S006120, C435S091200
Reexamination Certificate
active
07872116
ABSTRACT:
The present invention encompasses nucleic acids, methods, compositions, and kits for sensitive, rapid and specific detection ofMycoplasma, Acholeplasma, Ureaplasma, PhytoplasmaandSpiroplasmaspecies in a sample. The invention utilizes specific primers and amplification methods that permit differentiation between species due to specific amplification of target nucleic acids of contaminating Mollicute cells. In one embodiment, the invention utilizes the differing melting temperatures (Tm) of various potential PCR products to identify whether they are specific target amplification products, non-specific, non-target amplification products, specific positive control products, or primer-dimer products.
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patent: 2005/0250112 (2005-11-01), Padmabandu et al.
patent: WO 0123606 (2001-04-01), None
Volokhov, D.V. et al. Sequencing of the intergenic 16S-23S rRNA spacer (ITS) region of Mollicutes species and their identification using microarray-based assay and DNA sequencing. Appl. Microbiol. Biotechnol., vol. 71, pp. 680-698, published on line 10, Feb. 2006.
Lowe et al., A computer program for selection of oligonucleotide primers for polymerase chain reactions. Nucleic Acids Research, vol. 18, No. 7, pp. 1757-1761, 1990.
Cottrill Karin
Ikonomi Pranvera
Li Quanyi
Polayes Deborah
American Type Culture Collection (ATCC)
Bio Intellectual proprty Services LLC (Bio IPS)
Chunduru Suryaprabha
Zaghmout O. M (Sam)
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