Identification of a DNA region potentially useful for the detect

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

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435 6, 435 691, 435 911, 536 243, 536 2432, C12N 112, C12Q 168, C07H 2104

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active

060135107

ABSTRACT:
Disclosed herein is a newly-identified DNA sequence from Mycobacterium kansasii designated KATS2. Also disclosed are methods, oligonucleotide probes, amplification primers, and kits for the detection of M. kansasii nucleic acids. M. kansasii-specific methods, probes, amplification primers, and kits are preferred.

REFERENCES:
T. Rogall, et al. "Differentiation of Mycobacterium species by direct sequencing of amplified DNA" J. Gen. Microbiol. 136:1915-1920 (1990).
B. Boddinghaus, et al. "Detection and Identification of Mycobacteria by Amplification of rRNA" J. Clin. Microbiol. 28:1751-1759 (1990).
M. Yang, et al. "Isolation of a DNA Probe for Identification of Mycobacterium kansasii, Including the Genetic Subgroup"J. Clin. Microbiol. 31:2769-2772 (1993).
E. Tortoli, et al. "Evaluation of a Commercial DNA Probe Assay for the Identification of Mycobacterium kansasii" J. Clin. Microbiol. Infe. Dis. 13:264-267 (1994).
B. C. Ross, et al. "Identification of a Genetically Distinct Subspecies of Mycobacterium kansasii" J. Clin. Microbiol. 30:2930-2933 (1992).
Z. H. Huang, et al. "Identification of Mycobacterium kansasii by DNA Hybridization" J. Clin. Microbiol. 29:2125-2129 (1991).
Philipp et al, "An integrated map of the genome of the tubercule bacillus, Mycobacterium tuberculosis H37Rv, and comparison with Mycobacterium leprae", Proc. Natl. Acad. Sci., USA. vol. 93, pp. 3132-3137, Apr. 1, 1996.
EMBL55 database, Accession No. Z94121, MPSRCH, n.a. database search, Apr. 15, 1997

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