Identification of a CD34+ bone marrow precursor for dendritic ce

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 2, 435325, 435355, 435372, 436824, 53038822, G01N 3353, C12N 508, C07K 1628

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060080043

ABSTRACT:
Dendritic cells (DCs) are the primary antigen presenting cells during the initiation of T cell-dependent immune responses. The cells originate from the bone marrow and have been suggested to represent a distinct cell lineage. However, distinct DC precursors have not been identified in bone marrow, and mature monocytes can also give rise to DCs. The instant invention presents a distinct DC precursor among bone marrow CD34+ cells. The cells express high levels of the interleukin-3 receptor a chain and CD4 and can be uniquely identified also in blood and lymphoid tissues by this phenotype.

REFERENCES:
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Galy, A. et al. Immunity 3: 459-473, Oct. 1995.
Santiago-Schwarz, F. et al. Blood 82(10): 3019-3028, Nov. 1993.
C. Caux et al.: "Interleukin-3 cooperates with tumor necrosis factor alpha for the development of human dendritic.sub.- Langerhans cells from cord blood CD34+ hematopoietic progenitor cells." Blood, vol. 87, No. 6, Mar. 15, 1996, New York, NY, USA, pp. 2376-2385, XP002052034 See the whole document.
B. Herbst et al.: "In vitro differentiation of CD34+ hematopoietic progenitor cells toward distinct dendritic cell subsets of the birbeck granule and MIIC-positive Langerhans cell and the interdigitating dendritic cell type." Blood vol. 88, No. 7, Oct. 1, 1996, New York, NY, USA, pp. 2541-2548, XP002052035 see the whole document.
W. Egner et al.: "The phenotype of freshly isolated and cultured human bone marrow allostimulatory cells: possible heterogeneity in bone marrow dendritic cell populations." Immunology, vol. 85, No. 4, Aug. 1995, Oxford, GB, pp. 611-620, XP000608115 see abstract.

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