Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1991-08-26
1992-12-01
Patterson, Jr., Charles L.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
4352533, 435271, C12N 920
Patent
active
051680602
ABSTRACT:
A novel lipase from a newly-discovered strain of Pseudomonas alcaligenes microorganism having (i) an optimum pH for activity of about 10.+-.0.5; (ii) an optimum temperature for activity of about 45.degree. to 55.degree. C.; (iii) an optimum pH for stability of about 7.0.+-.0.5; (iv) a molecular weight as measured by SDS-PAGE of about 3.0.times.10.sup.4 ; and (v) chemical stability for at least a 60 day mean half-life in the presence of a 10 percent solution of polyoxyethylene (23) lauryl ether in 25 millimolar aqueous calcium chloride. Also claimed is a biologically pure culture of the microorganism, and a method for the production of the lipase.
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Ralston-Barrett, E., N. J. Palleroni and M. Doudoroff, Inter, J. System. Bacteriol, 26(4): 421-426 (1976).
ATCC Catalogue of Bacteria and Phages (17th Ed) 1989, p. 170.
Watanabe, N. et al., (1977) Agril. Biol Chem, 41(8), 1353-1358.
Carlson Dale Lynn
Olin Corporation
Patterson Jr. Charles L.
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