Identification and use of genes encoding holins and...

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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Reexamination Certificate



This invention provides: 1) methods for the identification of broad-spectrum holins with a high level of nonenzymatic activity in membranes; 2) conditions required for maintaining and increasing the anti-microbial and anti-pest efficacy of holins in gene fusions; 3) a method for effective targeting of holins expressed in plants through use of a leader peptide to direct the holin protein to the plant apoplast and xylem; 4) methods for the control of bacterial and fungal diseases of plants and control of insect and nematode pests that attack plants by expression of gene fusions involving holins, C-terminal additions and leader peptides, and optionally, endolysins; 5) methods for increasing the shelf-life of cut flowers, and 6) transgenic plants useful for the production of novel antimicrobial proteins based upon holins.

patent: 6783930 (2004-08-01), Pelletier et al.
patent: 6858707 (2005-02-01), Wei et al.
patent: 2009/0036307 (2009-02-01), Gabriel et al.
Grundling et al., Dimerization between the Holin and Holin Inhibitor of Phage Lambda, Journal of Virology, 2000, 182(21):6075-6081.
Masaya Oki, et al., “Functional and structural features of the holing HOL protein of theLactobacillus plantarumphage Φgle: analysis inEscherichia colisystem”, GENE 197 (1997) pp. 137-145.


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