Identification and use of antiviral compounds that inhibit inter

Details Identification and use of antiviral compounds that inhibit inter Identification and use of antiviral compounds that inhibit inter

1994-05-20

1998-05-12

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

4353201, 435325, 536 235, C12N 121, C12N 1563, C12N 510, C07H 2104

Patent

active

057503940

ABSTRACT:
The present invention relates to the identification of host cell proteins that interact with viral proteins required for virus replication, and high throughput assays to identify compounds that interfere with the specific interaction between the viral and host cell protein. Interfering compounds that inhibit viral replication can be used therapeutically to treat viral infection.
The invention is based, in part, on the discovery described herein of a novel interaction between the NP of influenza virus and a human host cell protein. The host cell protein, referred to herein as NPI-1, may be an accessory protein required for replication of influenza virus. Compounds that interfere with the binding of the host cell and viral proteins, and inhibit viral replication can be useful for treating viral infection in vivo.

REFERENCES:
Scholtissek, et al., 1985, Virology 147: 287-294.
Bean, 1984, Virology 133: 438-442.
Buckler-White & Murphy, 1986, Virology 155: 345-355.
Gammelin, et al. 1989, Virology 170:71-80.
Scholtissek, et al. 1978, Virology 91: 79-85.
Huang, et al., 1990, J. Virol. 64: 5669-5673.
Beaton & Krug, 1986, Proc. Natl. Acad. Sci USA 83:6282-6286.
Shapiro & Krug, 1988, J. Virol. 62: 2285-2290.
Honda, et al., 1988, J. Biochem. 104: 1021-1026.
Barik & Banerjee, 1992, Proc. Natl. Acad. Sci. USA 89: 6570-6574.
Barik & Banerjee, 1992, J. Virol. 66: 1109-1118.
Lahiri & Thomas, 1986, Nucl. Acids Res. 14: 4077-4094.
Chelsky et al., 1989, "Sequence requirements for synthetic peptide-mediated translocation to the nucleus", Mol. Cell. Biol. 9:2487-2492.
Chien et al., 1991, "The two-hybrid system: A method to identify and clone genes for proteins that interact with a protein of interest", PNAS USA 88:9578-9582.
Cuomo et al., 1994, Mtg abstr. F015, Keystone Symp. on Recombination.
Dalton & Treisman, 1992, "Characterization of SAP-1, a protein recruited by serum response factor to the c-fos serum response element", Cell 68:597-612.
Durfee et al., 1993, "The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit", Genes Dev. 7:555-569.
Enami et al., 1990, "Introduction of site-specific mutations into the genome of influenza virus", PNAS USA 87:3802-3805.
Gyuris et al., 1993, "Cdi1, a human G1 and S phase protein phosphatase that associates with Cdk2", Cell 75:791-803.
Hall et al., 1984, "Targeting of E. coli .beta.-Galactosidase to the nucleus in yeast", Cell 36:1057-1065.
Jackson et al., 1982, "Influenza virus RNA is synthesized at fixed sites in the nucleus", Nature 296:366-368.
McCrea et al., 1991, "A homolog of the armadillo protein in Drosophila (Plakoglobin) associated with E-Cadherin", Science 254:1359-1361.
Parvin et al., 1989, "Promoter analysis of influenza virus RNA polymerase", J. Virol. 63:5142-5152.
Peifer et al., 1994, "A repeating amino acid motif shared by proteins with diverse cellular roles", Cell 76:789-791.
Riggleman et al., 1989, "Molecular analysis of the armadillo locus: Uniformly distributed transcripts and a protein with novel internal repeats are associated with a Drosophila segment polarity gene", Genes & Dev. 3:96-113.
Vojtek et al., 1993, "Mammalian Ras interacts directly with the Serine/Threonine kinase Raf", Cell 74:205-214.
Yano et al., 1992, "Cloning and characterization of SRP1, a suppressor of temperature-sensitive RNA polymerase l mutations, in Saccharomyces cerervisiae", Mol. Cell. Biol. 12:5640-5651.
Zervos et al., 1993, "Mxi1, a protein that specifically interacts with Max to bind Myc-Max recognition sites", Cell 72:222-232.
Baudin et al., 1994, "Structure of influenza virus RNP. 1. Influenza virus nucleoprotein melts secondary structure in panhandle RNA and exposes the bases to the solvent", EMBO J. 13:3158-3165.
Joklik et al. (eds.), 1992, "Antiviral chemotherapy, interferon, and vaccines", Zinsser Microbiology Appleton & Lange, Norwalk, Conn. Chap. 58, pp. 854-861.
Nakada et al., 1984, "Complete nucleotide sequence of the influenza C/California/78 virus nucleoprotein gene", Virus Res. 1:433-441.
Yano, R. et al. (1992) "Cloning and characterization of SRP1, a suppressor of temperature-sensitive RNA polymerase l mutations, in Saccharomyces cerevisiae" Mol. Cell. Biol. 12(12):5640-5651.

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