Identification and sorting of plant heterokaryons

Details Identification and sorting of plant heterokaryons Identification and sorting of plant heterokaryons

1980-07-28

1981-11-17

Bagwill, Robert E.

Plant husbandry

Process

A01H 102

Patent

active

043003109

ABSTRACT:
To label heterokaryons formed by fusion of different somatic tissue cells, somatic tissue cells from one plant are immersed in a first solution including polysaccharidase and fluorescein isothiocyanate and somatic tissue cells from a different plant are immersed in a second solution including polysaccharidase and rhodamine isothiocyanate for a period of from 12 to 18 hours, after which protoplasts emerge, with those from one plant fluorescing green-yellow and those from the other fluorescing orange-red. The protoplasts are then fused in a polyethylene glycol solution. The heterokaryons are identified and sorted by a commercial cell sorter which relies upon the fluorescence from both markers.

REFERENCES:
patent: 3832801 (1974-09-01), Carlson et al.
Plant Cell, Tissue, and Organ Culture, Reinert et al., 1977, Springer-Verlag, N.Y., pp. 467-505.

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