Identification and functional characterization of a novel...

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease

Reexamination Certificate

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C435S015000, C435S320100, C435S069100, C530S350000, C536S023200, C536S023500

Reexamination Certificate

active

07998718

ABSTRACT:
A novel S6 kinase, p70βS6kis described, along with methods of making and using p70βS6kprotein and related nucleic acids. The invention also discloses methods of identifying agents which modulate the activity of p70βS6kprotein and/or its ligands.

REFERENCES:
patent: 5932445 (1999-08-01), Lal et al.
patent: 6156523 (2000-12-01), Bandman et al.
patent: 6372467 (2002-04-01), Blenis et al.
patent: WO 9319752 (1993-10-01), None
patent: WO 9803662 (1998-01-01), None
patent: WO 9818935 (1998-05-01), None
patent: WO 9924463 (1999-05-01), None
Branden et al., Introduction to Protein Structure, Garland Publishing Inc., New York, p. 247, 1991.
Alessi, D.R.,The Protein Kinase Inhibitors Ro 318220 and GF 10903X are equally potent inhibitors of MAPKAP kinase-1 beta(Rsk-2), FEBS Lett. 402:121-3 (1997).
Alessi et al.,3-Phosphoinositide-Dependent Protein Kinase 1(PDKI)Phosphorylates and Activates the p70 S6 Kinase In Vivo and In Vitro, Current Biol. 8:69-81 (1997).
Boluyt et al.,Rapamycin Inhibits Alpha 1-adrenergic Receptor-stimulated cardiac myocyte hypertrophy but not activation of hypertrophy-associated genes. Evidence for involvement of p70 S6 kinase, Circ. Res. 81:176-186 (1997).
Buckle et al.,HTLV-I-induced T-cell activation, J. Acquir. Immune. Defic. Synd. Hum. Retrovirol. 13 Suppl. 1: S107-13 (1996).
Busca et al.,Inhibition of the Phosphatidylinositol 3-kinase/p70(S6)kinase Pathway Induces B16 Melanoma Cell Differentiation, J. Biol. Chem. 271:31824-31830 (1996).
Coolican et al.,The miogenic and myogenic actions of insulin-like growth factors utilize distinct signaling pathways, J. Biol. Chem. 272:6653-6662 (1997).
Crawley et al.,Interleukin-10 Stimulation of Phosphatidylinositol 3-kinase and P70 S6 Kinase Is Required for the Proliferative but Not the Antiinflammatory Effects of the Cytokine, J. Biol. Chem. 271:16357-16362 (1996).
Dennis et al.,The Principal Rapamycin-Sensitive p7056KPhosphorylation Sites, T-229 and T-389, are Differentially Regulated by Rapamycin-Insensitive Kinase Kinases, Mol. and Cell. Biol. 16:6242-6251 (1996).
Gout et al.,Molecular Cloning and Characterization of a Novel p70 S6 Kinase Containing a Proline-Rich Region, J. Biol. Chem. 273:30061-30064 (1998).
Grove et al.,Cloning and Expression of Two Human P70 S6 kinase Polypeptides Differing Only at Their Amino Termini, Mol. Cell. Biol. 11:5541-5550 (1991).
Flamigni et al.,Phosphatidylinositol 3-Kinase is Required for the Induction of Ornithine Decarboxylase in Leukemia Cells Stimulated to Growth, Biochem Biopphys Res Comm 239: 729-33 (1997).
Han et al.,Rapamycin, Worthmannin and the Methylxanthine SQ20006 Inactivate p70S6K by Inducing Dephosphorylation of the Same Subset of Sites, J. Biol. Chem. 270:21396-21403 (1995).
Hara et al.,Regulation of eIF-4E BP1 Phosphorylation by mTOR, J. Biol. Chem. 272: 26457-63 (1997).
Kanda et al.,Phosphatidylinositol 3-Kinase-independent P70 S6 Kinase Activation by Fibroblast Growth Factor Receptor-1 Is Important for Proliferation but Not Differentiation of Endothelial Cells, J. Biol. Chem. 272: 23347-23353 (1997).
Kawamata et al.,The Upregulation of p27Kip1 by Rapamycin Results in GI Arrest in Exponentially growing T cell lines, Blood 19: 561-9 (1998).
Kinoshita et al.,Raf/MAPK and Rapamycin-sensitive Pathways Mediate the Anti-apoptotic Function of p2lRas in IL-3-dependent Hematopoietic Cells, Oncogene 15: 619-27 (1997).
Koyama et al.,Fibrillar Collagen Inhibits Arterial Smooth Muscle Proliferation Through Regulation of Cdk2 Inhibitors, Cell 87:1069-1078 (1996).
Leighton et al.,Comparison of the Specificities of p70 S6 Kinase and MAPKAP Kinase-1 identifies a relatively specific substrate for p70 S6 kinase: the N-terminal Kinase Domain of MAPKAP Kinase-I is essential for Peptide Phosphorylation, FEBS Letters 375: 289-293 (1995).
Lin et al.,Heat Shock Activates c-Src tyrosine Kinases and Phosphatidylinoitol 3-kinase in NIH3T3 Fibroblasts, J. Biol. Chem. 272:31196-31202 (1997).
Morreale et al.,Ro31-8220 Inhibits Protein Kinase C to Block the Phorbol Ester-stimulated Release of Choline and Ethanolamine-metabolites from C6 Glioma Cells: p70 S6 Kinase and MAPKAP Kinase-Ibeta Do Not Function Downstream of PKC in Activating PLD, FEBS Letters 417:38-42 (1997).
Mukhopadhyay et al.,An Array of Insulin-Activated, Proline-Directed Serine/Threonine Kinases Phosphorylate the p70 S6 Kinase, J. Biol. Chem. 267:3325-3335 (1995).
Pai et al.,Cross-linking CD28 leads to activation of 7-kDa S6 Kinase, Eur. J. Immunol. 24:2364-2368 (1994).
Pearson et al.,The Principal Target of Rapamycin-Induced p70S6K Inactivation is a Novel Phosphorylation Site Within a Conserved Hydrophobic Domain, EMBO J. 14:5279-5287 (1995).
Petritsch et al.,Selective Inhibition of p70 S6 Kinase Activation by Phosphatidylinositol 3-Kinase Inhibitors, Eur. J. Biochem. 230:431-438 (1995).
Proud,P70 S6 Kinase: an Engima With Variations, TIBS Trends in Biochem. 21:181-185 (1996).
Pullen et al.,The Modular Phosphorylation and Activation of p70s6k, FEBS Letters 410:78-82 (1997).
Saitoh et al.,Cloning and Characterization of p70S6Defines a Novel Family of p70 S6 Kinases, Biochem. Biophys. Res. Commun. 253: 471-476 (1998).
Stewart et al.,Mitogenesis and Protein Synthesis: a role for Ribosomal Protein S6 Phosphorylation, BioEssays 16: 809-815 (1994).
Weng et al.,Regulation of the p70 S6 Kinase by Phosphorylation In Vivo, J. Biol. Chem. 273: 16621-16629 (1998).
Lee-Fruman et al.,Characterization of S6K2, a novel kinase homologous to S6K1, Oncogene 18: 5108-5114 (1999).
Bork,Powers and pitfalls in sequence analysis: the 70%hurdle, Genome Research 10: 398-400 (2000).
Broun et al.,Catalytic plasticity of fatty acid modification enzymes underlying chemical diversity of plant lipids, Science 282: 1315-1317 (1998).
Seffernick et al.,Melamine deaminase and atrazine chlorohydrolase: 98 percent identical but functionally different, J. Bacteriol. 183: 2405-2410 (2001).
Van de Loo et al.,An oleate 12-hydroxylase from Ricinus communis L. is a fatty acyl desaturase homolog, Proc. Natl. Acad. Sci: 92: 6743-6747 (1995).
Witkowski et al.,Conversion of a beta-ketoacyl synthase to a malonyl decarboxylase by replacement of the active-site cysteine with glutamine, Biochemistry, 38: 11643-11650 (1999).

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