Identification and enumeration of microbial cells

Details Identification and enumeration of microbial cells Identification and enumeration of microbial cells

1982-04-26

1984-08-21

Naff, David M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 29, 435 32, 435 39, 435291, C12Q 104, C12Q 129, C12Q 106, C12M 134

Patent

active

044670320

ABSTRACT:
A process and apparatus is disclosed for rapid and accurate identifying and enumerating of microorganisms. A sample believed to contain a certain microorganism is combined with an inhibiting reagent having a known specificity for the microorganism. The combined sample and inhibiting reagent is placed in an alternating current electrical field having a frequency sufficiently high to cause alignment of microbial cells in the sample. A polarized beam of laser light is then passed through the sample and the extent of birefringence is measured. The measured birefringence is compared with that of a control sample known to contain the microorganism under investigation to determine the microorganism in the sample.

REFERENCES:
patent: 4101383 (1978-07-01), Wyatt et al.
Morris et al., The Effect of Neomycin and Streptomycin on the Electrical Polarisability of Aqueous Suspensions of Escherichia cd., Biochimica et Biophysica Acta, vol. 392, 1975, (pp. 328-334).
Morris et al., The Effect of Antibiotics on the Electrical Polarisability of Aqueous Suspensions of Bacteria, Biochimica et Biophysica Acta, vol. 497, 1977, (pp. 253-259).

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