Identification and comparison of protein-protein interactions th

Details Identification and comparison of protein-protein interactions th Identification and comparison of protein-protein interactions th

1996-06-14

2000-07-04

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 691, 435 697, 435 701, 435 711, 43525411, 4351723, 935 77, 935 78, C12Q 168, C12P 2102, C12N 115, C12N 1563

Patent

active

060836938

ABSTRACT:
Methods are described for detecting protein--protein interactions, among two populations of proteins, each having a complexity of at least 1,000. For example, proteins are fused either to the DNA-binding domain of a transcriptional activator or to the activation domain of a transcriptional activator. Two yeast strains, of the opposite mating type and carrying one type each of the fusion proteins are mated together. Productive interactions between the two halves due to proten--protein interactions lead to the reconstitution of the transcriptional activator, which in turn leads to the activation of a reporter gene containing a binding site for the DNA-binding domain. This analysis can be carried out for two or more populations of proteins. The differences in the genes encoding the proteins involved in the protein--protein interactions are characterized, thus leading to the identification of specific protein--protein interactions, and the genes encoding the interacting proteins, relevant to a particular tissue, stage or disease. Furthermore, inhibitors that interfere with these protein--protein interactions are identified by their ability to inactivate a reporter gene. The screening for such inhibitors can be in a multiplexed format where a set of inhibitors will be screened against a library of interactors.

REFERENCES:
patent: 4593002 (1986-06-01), Dulbecco
patent: 5223409 (1993-06-01), Ladner et al.
patent: 5270170 (1993-12-01), Schatz et al.
patent: 5283173 (1994-02-01), Fields et al.
patent: 5322801 (1994-06-01), Kingston et al.
patent: 5403712 (1995-04-01), Crabtree et al.
patent: 5468614 (1995-11-01), Fields et al.
patent: 5482835 (1996-01-01), King et al.
patent: 5512473 (1996-04-01), Brent et al.
patent: 5525490 (1996-06-01), Erickson et al.
patent: 5580721 (1996-12-01), Brent et al.
patent: 5580736 (1996-12-01), Brent et al.
LeDovariu et al. Nucl. Acids Res. 23: 876-878, Mar. 1995.
Mendelsohn et al. Current Opinion in Biotechnology 5: 482-486, Oct. 1994.
Alam and Cook, 1990, Anal. Biochem. 188:245-254.
Allen et al., 1995, TIBS 20:511-516.
Alvarez et al., 1996, Nature Biotech. 14:335-335-338.
Bartel et al., 1993, Oxford University Press, NY, (Hartley, ed.), pp. 153-179.
Bartel et al., 1993, BioTechniques 14(6):920-924.
Bartel et al., 1996, Nature Genetics 12:72-77.
Bendixen et al., 1994, Nucl. Acids Res. 22(9):1778-1779.
Bonner et al., 1986, Nucl. Acids Res. 14(2):1009-1015.
Bustos and Schleif, 1993, Proc. Natl. Acad. Sci. USA 90:5638-5642.
Celenza and Carlson, 1986, Science 233:1175-1180.
Chaudhuri and Stephan, 1995, Biochem. Biophys. Res. Comm. 215(2):781-790.
Chaudhuri et al., 1995, FEBS Lett. 357:221-226.
Chien et al., 1991, Proc. Natl. Acad. Sci. USA 88:9578-9582.
Chuang et al., 1994, Mol. Cell. Biol. 14(8):5318-5325.
Claffey et al., 1995, Biochim. et Biophys. Acta 1246:1-9.
Coney and Roeder, 1988, Mol. Cell. Biol. 8(10):4009-4017.
Colas et al., 1996, Nature 380:848-850.
Dingwall and Laskey, 1991, TIBS 16:479-481.
Dower, 1992, Current Biol. 2(5):251-253.
Durfee et al., 1993, Genes & Dev. 7:555-569.
Elledge and Davis, 1988, Gene 70:303-312.
Estojak et al., 1995, Mol. Cell. Biol. 15(10):5820-5829.
Fearon et al., 1992, Proc. Natl. Acad. Sci. USA 89:7958-7962.
Fields and Song, 1989, Nature 340:245-246.
Fields and Sternglanz, 1994, TIG 10(8):286-292.
Finley and Brent, 1994, Proc. Natl. Acad. Sci. USA 91:12980-12984.
Finley and Brent, in Gene Probes: A Practical Approach, Hames and Glover (eds.), Oxford University Press, Oxford, pp. 169-203.
Flam, 1994, Science 266:1324-1325.
FluoReporter.RTM. lacZ/Galactosidase Quantitation Kit (F-2905), 1995, Molecular Probes, Inc., pp. 1-4.
Franzen et al., 1993, Cell 75:681-692.
Fleig et al., 1986, Gene 46:237-245.
Galacto-Light.TM., Galacto-Light Plus.TM., 1995, Tropix, Inc., pp. 1-6.
Germino et al., 1993, Proc. Natl. Acad. Sci. USA 90:933-937.
Green et al., 1995, Chem. & Biol. 2:683-695.
Guarente, 1993, Proc. Natl. Acad. Sci. USA 90:1639-1641.
Gyuris et al., 1993, Cell 75:791-803.
Harper et al., 1993, Cell 75:805-816.
Heil, 1993, Gen. Engin. News 13(14):1-2.
Herskowitz and Jensen, 1991, Meth. Enzymol. 194:132-146.
Hong et al., 1994, Yeast 10:1083-1092.
Hu et al., 1990, Science 250:1400-1403.
Hu, 1995, Structure 3:431-433.
Hutchinson, 1994, Bio/Technology 12:375-380.
HybriZAP.TM. Two-Hybrid cDNA Gigapack.RTM. Cloning Kit, Instruction Manual, 1995, Stratagene, pp. 1-73.
Ito et al., 1983, J. Bacteriol. 153(1):163-168.
Jain and Magrath, 1991, Anal. Biochem. 199:119-124.
Jellinek et al., 1994, Biochemistry 33:10450-10456.
Johnston, 1987, Microbiol. Rev. 51(4):458-476.
Kato, 1996, Nucl. Acids Res. 24(2):394-395.
Keegan et al., 1986, Science 231:699-703.
Kishore and Shah, 1988, Ann. Rev. Biochem. 57:627-663.
Le Douarin et al., 1995, Nucl. Acids Res. 23(5):876-878.
Leung et al., 1989, Science 246:1306-1309.
Li and Herskowitz, 1993, Science 262:1870-1874.
Milosavljevic et al., 1996, Genome Res. 6:132-141.
Miura et al., 1986, Jpn. J. Cancer Res. (Gann) 77:45-51.
Murray et al., 1993, Gene 134:123-128.
Nallur et al., 1993, Nucl. Acids. Res. 21(16)3867-3873.
Nature Biotechnology, 1996, 14:247.
Potgens et al., 1994, J. Biol. Chem. 269(52):32879-32885.
Prashar and Weissman, 1996, Proc. Natl. Acad. Sci. USA 93:659-663.
Rose and Botstein, 1983, Meth. Enzymol. 101:167-180.
Rothstein, 1991, Meth. Enzymol. 194:281-301.
Schneider and Guarente, 1991, Meth. Enzymol. 194:373-388.
Schmidt-Dorr et al., 1991, Biochem. 30:9657-9664.
Sikorski and Boeke, 1991, Meth. Enzymol. 194:302-318.
Stratford, 1994, Yeast 10:1741-1752.
Struhl et al., 1979, Proc. Natl. Acad. Sci. USA 76:1035-1039.
Terman et al., 1992, Biochem Biophys. Res. Comm. 187(3):1579-1586.
Wang et al., 1994, Science 265:674-676.
Wang et al., 1996, Science 271:1120-1122.
Vasavada et al., 1991, Proc. Natl. Acad. Sci. USA 88:10686-10690.
Velculescu et al., 1995, Science 270:484-487.
Yang et al., 1995, Nucl. Acids. Res. 23(7):1152-1156.
Yocum et al., 1984, Mol. Cell. Biol. 4(10):1985-1998.

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