Identification and cloning of a mycobacterial antigen...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C424S009100, C424S130100, C424S141100, C424S150100, C424S164100, C424S168100, C424S184100, C424S185100, C424S190100, C424S200100, C424S248100, C435S004000, C435S007200, C435S007320, C435S041000, C435S042000, C435S440000, C435S471000, C530S300000, C530S350000, C536S023100, C536S023700

Reexamination Certificate

active

06949345

ABSTRACT:
The present invention relates to peptide sequences enabling mycobacteria to adhere to host cells (e.g., epithelial cells). More particularly, the invention relates to a mycobacterial heparin-binding haemagglutinin type antigen fromM. bovisECG orM. tuberculosis. The invention also relates to a recombinant peptide sequence enabling mycobacteria to adhere to host cells. The polypeptides can be used to prepare vaccines against mycobacterial infections and for serological diagnosis of mycobacterial infections.

REFERENCES:
Menozzi et al, “A Heparin-Binding Hemagglutinin in mycobacteria”, Abstracts of the General Meeting of the American Society for Microbiology 95(0):193 (1995)—Abstract B-159 & 95thGeneral Meeting of the American Society for Microbiology, Washington, DC USA, May 21-25, 1995.
Brennan et al, “Identification of a Heparin-Binding Mycobacterial Hemagglutinin”, Journal of Cellular Biochemistry Supplement 0 (19B), p. 66 (1995)—Abstract B3-104 & Keystone Symposium on Molecular Mechanisms in Tuberculosis, Tamarron, Colorado, USA, Feb. 19-25, 1995.
Isaacs, “Borrelia burgdorferiBind to Epithelial Cell Proteoglycans”, Journal of Clinical Investigation 93:809-819 (1994).
Menozzi et al, “Heparin-Inhibitable Lectin Activity of the Filamentous Hemagglutinin Adhesin ofBordetella pertussis”, Infection and Immunity 62(3):769-778 (1994).
Hudson et al, “Comparison of the Fibronectin-binding Ability and Antitumor Efficacy of Various Mycobacteria”, Cancer Research 50:3843-3847 (1990).
Rouse et al, “Immunological Characterization of Recombinant Antigens Isolated from aMycobacterium aviumλgt11 Expression Library by Using Monoclonal Antibody Probes”, Infection and Immunity 59(8):2595-2600 (1991).
“Mycobacterium tuberculosiscosmid SCY20G9”, Banque De Donnees Embl, Identificateur MTCY20G9, Accession No. Z77162, Sep. 18, 1996.
Menozzi et al, “Identification of a Heparin-binding Hemagglutinin Present in Mycobacteria”, Journal of Experimental Medicine 184(3):993-1001 (1996).

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