Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1998-04-15
1999-12-21
Chin, Christopher L.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 732, 514 44, C12N 1500
Patent
active
060043547
ABSTRACT:
A purified IceA protein of Helicobacter pylori is provided. The protein is expressed as either an IceA 1 or an IceA 2 variant. A purified polypeptide fragment of the IceA protein is also provided. An antigenic fragment of IceA is provided. An isolated nucleic acid that encodes an IceA protein of H. pylori is provided. A nucleic acid that encodes an IceA 1 variant and a nucleic acid that encodes an IceA 2 variant is also provided. Fragments of the iceA gene are provided. A method of detecting the presence of an antibody against H. pylori in a sample is provided. The method comprises the following steps: a) contacting the sample with a purified IceA protein of H. pylori or a H. pylori-specific fragment thereof; and b) detecting the binding of the antibody in the sample to the protein or fragment, the detection of binding indicating the presence in the sample of antibodies against H. pylori. A method of detecting the presence of an antibody against an ulcerative Helicobacter pylori strain in a sample is also provided.
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Blaser Martin J.
Miller Geraldine G.
Peek, Jr. Richard M.
Thompson Stuart A.
Chin Christopher L.
Portner Ginny Allen
Vanderbilt University
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