Ice seeding apparatus for cryopreservation systems

Details Ice seeding apparatus for cryopreservation systems Ice seeding apparatus for cryopreservation systems

1996-01-30

1997-11-25

Capossela, Ronald C.

Refrigeration

Processes

Deodorizing, antisepticizing or providing special atmosphere

62373, F24F 316

Patent

active

056899610

ABSTRACT:
An apparatus for ice seeding during cryopreservation of biological samples such as cells, harvested tissues, and cellular biological constructs such as culture tissue equivalents wherein ice seeding is performed by discharging a liquefied or chilled gas from a nozzle to the surface container containing said biological sample in cryopreservative. Inside a freezing chamber, containers are placed on racks that are located proximal to a sprayrail containing nozzles. Ice seeding is performed by discharge of liquefied or chilled gas from the nozzles to the container surface at the solid-liquid equilibrium temperature of the cryopreservative. Localized cooling of the container surface by the discharge results in ice seed formation within the container.

REFERENCES:
patent: 3905206 (1975-09-01), Deubel et al.
patent: 4107937 (1978-08-01), Chmiel
patent: 4416380 (1983-11-01), Flum
patent: 5309722 (1994-05-01), Phillips, Jr.

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