Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Patent
1985-12-04
1988-08-23
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
4351721, 4351723, 435910, 435874, 435875, 435876, 435847, 424 93, 47 58, C12N 1500, C12N 120
Patent
active
047660775
ABSTRACT:
Ice nucleation bacteria are modified in vitro to confer an ice nucleation deficient phenotype. Modification is accomplished by deletion, substitution, insertion, inversion, or transversion of a DNA segment within the gene locus responsible for the INA phenotype. By limiting such mutations to the particular gene locus, the modified microorganisms are genetically stable and free from random mutations which might adversely affect their competitive fitness. The modified microorganisms are useful for prevention of frost damage to susceptible plant hosts.
REFERENCES:
patent: 4161084 (1979-06-01), Arny et al.
patent: 4375734 (1983-03-01), Kozloff et al.
patent: 4464473 (1984-08-01), Orser et al.
patent: 4706463 (1987-11-01), Lindsey
Orser et al., Phytopathology, vol. 72(7), 1982, p. 1000, "Cloning and Expression of Ice Nucleation Genes from Pseudomonas syringae and Erwinia herbicola.
Lindow et al., Phytopathology, vol. 71(2), 1981, p. 237, "Isolation of Ice Nucleation Deficient Mutants of Pseudomonas syringae and Erwinia herbicola and their Transformation with Plasmid DNA.
Lindow Steven
Orser Cindy S.
Panapoulos Nickolas J.
Rowland Bertram I.
Terlizzi Laura
Teskin Robin Lyn
The Regents of the University of California
Wiseman Thomas G.
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