Ice-mediated introduction of substances into biological material

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

4351721, 4352404, 935 52, 935 53, 935 85, 239 142, C12N 1587, C12N 1589, C12N 1590

Patent

active

052197468

ABSTRACT:
Methods and apparatus for the introduction of foreign substances, such as recombinant DNA, into living cell interiors is disclosed. The desired substance to be introduced is dissolved or suspended in water or another suitable liquid to form a solution which is then nebulized and frozen to produce ice particles. These ice particles are accelerated toward and impact upon a target tissue where some of the particles impregnate at least some cells in the target tissue without killing the cells. Following impregnation, the ice particles melt, leaving behind the desired substance in the protoplasm of the bombarded cell.

REFERENCES:
patent: 4945050 (1990-07-01), Sanford et al.
Sanford et al. 1987 Particulate Science and Technology 5:27-37.
Klein et al. 1987, Science, 327: 70-73.
Criston et al. 1989 Proc. Natl. Acad. Sci. USA 86:7500-7504.
Potrykus 1990 (June) Bio/Technology 8:535-542.
Biolistic Particle Delivery Systems, Bibliography, Bio-Rad Genetic Systems News.
Transform Virtually Anything!, Bio-Radiations; A Life Science Research Products Newsletters; 1991; No. 80.
S. A. Johnston, M. Riedy, M. J. DeVit, J. C. Sanfort, S. McElligott and R. S. Williams; Plant; In Vitro Cellular & Development Biology; vol. 27P, No. 1; 1990-1991.
S. A. Johnston; Biolistic Transformation: Microbes to Mice; Nature; vol. 346: pp. 776-777 Aug. 23, 1990.
Richard M. Baldarelli and Judity A. Lengyel; Transient Expression of DNA after Ballistic Introduction into Drosophila Embryos; Nucleic Acids Research, vol. 18: No. 19: pp. 5903-5904; Aug. 27, 1990.
Karen L. Kindle, Rogene A. Schnell, Emilio Fernandez and Paul A. Lefebvre; Stable Nuclear Transformation of Chlamydomonas Using the Chlamydomonas Gene for Nitrate Reductase; The Journal of Cell Biology, vol. 109 (No. 6, Pt 1) p. 2589; Dec. 1989.
Carole Gan; Gene Gun Accelerates DNA-Coated Particles to Transform Intact Cells; The Scientist, Sep. 18, 1989, p. 25.
Roger Segelken; $2.28 Million Agreement Transfers Gene Gun Technology to DuPont; Cornell Agriculture & Life Sciences News; Sep. 1989, p. 7.
John C. Sanford: The Biolistic Process; Reviews, Dec. 1988, vol. 6 pp. 299-302.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Ice-mediated introduction of substances into biological material does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Ice-mediated introduction of substances into biological material, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Ice-mediated introduction of substances into biological material will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1041973

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.