I Sce I-induced gene replacement and gene conversion in embryoni

Details I Sce I-induced gene replacement and gene conversion in embryoni I Sce I-induced gene replacement and gene conversion in embryoni

1996-08-07

1998-11-03

Ketter, James

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435354, 536235, C12N 1500

Patent

active

058307290

ABSTRACT:
This invention relates to a method of constructing a villin gene hybrid by inserting an I-Sce I restriction site next to or within a gene or cDNA encoding a villin protein. The insertion site of the I-Sce I restriction site is chosen as to provide a first downstream part and a second upstream part from the site, containing at least twelve nucleotides of the gene or cDNA encoding the villin protein. Furthermore, the insertion of the restriction permits a high frequency of homologous recombination events. The villin gene hybrid may be used to transfect eukaryotic cells, and particularly, embryonic stem cells.

REFERENCES:
patent: 5474896 (1995-12-01), Dujon et al.
Rousseau-Merck, et al., Human Genetics, vol. 78, pp. 130-133, 1988.

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