Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1995-06-14
1998-04-28
Prouty, Rebecca E.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
4351723, 4352523, 4353201, 435 711, 435 712, 536 231, 536 232, 935 14, 935 78, C12N 938, C12N 1556
Patent
active
057443459
ABSTRACT:
An isolated hyperthermostable .beta.-galactosidase gene derived from Pyrococcus furiosus is described. Examples include DNA sequences shown in SEQ ID NO. 2 and SEQ ID NO. 4 and DNAs hybridizable with these DNAs. A method of cloning the hyperthermostable .beta.-galactosidase gene in which each of the above sequences or portions thereof is used as a probe or primer is described. A process for producing a hyperthermostable .beta.-galactosidase by culturing a transformant into which a plasmid containing each of the above genes has been introduced is described. A particularly preferred gene encodes an SDS-resistant hyperthermostable .beta.-galactosidase.
REFERENCES:
patent: 5242817 (1993-09-01), Kelly et al.
patent: 5283189 (1994-02-01), Takase et al.
FEMS Microbiology Letters, vol. 109, 1993 pp. 131-138, Josef Gabelsberger et al. "Cloning and characterization of beta-glucoside hydrolysing enzymes of thermotoga maritima", p. 131, right column, paragraph 2-p. 132, left column, paragraph 2, p. 133, left column, paragraph 2-page 134, right column, paragraph 1.
Journal of Applied Biochemistry, vol. 2, No. 5, 1980, pp. 390-397, Vincenzo Buonocore et al., "A constitutive beta-galactosidase from the extreme thermoacidophile archaebacterium caldariella acidophia: Properties of the enzyme in the free state and immobilized whole cells", abstract, p. 390, paragraph 2-p. 391, paragraph 1, p. 394, paragraph 1, p. 395, paragraph 4 -p. 396, paragraph 1.
International Journal of Biochemistry, vol. 5, 1974, pp. 629-632, Robert P. Erickson "Stability of Escherichia coli beta-galactosidase in sodium dodecyl sulfate" abstract, p. 629, left column, paragraph 1.
H.R. Constantino et al., "Purification and Characterization of an alpha-Glucosidase From a Hyperthermophilic Archaebacterim, Pyrococcus friosus, Exhibiting a Temperature Optimun of 105 to 115.degree. C.", J. Bacteriol. 172(7): 3654-3660, Jul. 1990.
F.M. Pisani et al., "Thermostable .beta.-Galactosidase From the Archaebacterium Sulfolobus solfataricus", Eur. J. Biochem. 187: 321-328, Jan. 1990.
Y. Koyama et al., "cloning of Alpha and .beta.-Galactosidase Genes From an Extreme Thermophile, Thermus Strain T2, and Their Expression in Thermus thermophilus HB27", Appl. Environ. Microbiol. 56(7); 2251-2254, Jul. 1990.
M.V. Cubellis et al., "Isolation and Sequencing of a New .beta.-Galactosidase-Encoding Archaebacterial Gene", Gene 94: 89-94, Sep. 1990.
Asada Kiyozo
Hashino Kimikazu
Kato Ikunoshin
Koyama Nobuto
Odate Miki
Prouty Rebecca E.
Takara Shuzo Co. Ltd.
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