Hyperthermostable .beta.-galactosidase gene, enzyme encoded ther

Details Hyperthermostable .beta.-galactosidase gene, enzyme encoded ther Hyperthermostable .beta.-galactosidase gene, enzyme encoded ther

1997-12-18

1999-10-05

Prouty, Rebecca E.

Chemistry: molecular biology and microbiology

Process of mutation, cell fusion, or genetic modification

435 6, 435207, 435471, 435476, 536 231, 536 232, C12N 1556, C12N 1510, C12N 1563

Patent

active

059623268

ABSTRACT:
An isolated SDS-resistant hyperthermostable .beta.-galactosidase gene derived from Pyrococcus furiosus. Examples thereof include genes respectively shown in SEQ ID NO: 1 and SEQ ID NO: 2 and genes each hybridizable with the above gene shown in SEQ ID NO: 1. A method of cloning the hyperthermostable .beta.-galactosidase gene in which each of the above genes or pacts thereof is used as a probe or primer. A process for producing a hyperthermostable .beta.-galactosidase by culturing a transformant into which a plasmid containing each of the above gene has been introduced.

REFERENCES:
patent: 3816259 (1974-06-01), Collinge et al.
patent: 3919049 (1975-11-01), Kiuchi et al.
patent: 4229539 (1980-10-01), Miwa et al.
patent: 5082785 (1992-01-01), Manning et al.
patent: 5242817 (1993-09-01), Kelly et al.
patent: 5283189 (1994-02-01), Takase et al.
Fems Microbiology Letters, vol. 109, 1993 pp. 131-138, Josef Gabelsberger et al. "Cloning and characterization of beta-galactoside and beta-glucoside hydrolysing enzymes of thermotoga maritima", p. 131, right column, paragraph 2--p. 132, left column, paragraph 2, p. 133, left column, paragraph 2--p. 134, right column, paragraph 1.
Journal of Applied Biochemistry, vol. 2, No. 5, 1980, pp. 390-397, Vincenzo Buonocore et al., "A constitutive beta-galactosidase from the extreme thermoacidophile archaebacterium caldariella acidophila: Properties of the enzyme in the free state and immobilized whole cells", abstract, p. 390, paragraph 2--p. 391, paragraph 1, p. 394, paragraph 1, p. 395, paragraph 4--p. 396, paragraph 1.
International Journal of Biochemistry, vol. 5, 1974, pp. 629-632, Robert P. Erickson "Stability of Escherichia coli beta-galatosidase in sodium dodecyl sulfate" abstract, p. 629, left column, paragraph 1.
Y. Koyama et al., "Cloning of Alpha and .beta.-Galactosidase Genes From an Extreme Thermophile, Thermus Strain T2, and Their Expression in Thermus Thermophilus HB27", Appl. Environ. Microbiol. 56(7); pp. 2251-2254.
M.V. Cubellis et al., "Isolation and Sequencing of a New .beta.-Galactosidase-Encoding Archaebacterial Gene", Gene, 94: pp. 89-94.
Critical Reviews in Food Technology, Jan. 1975, pp. 330-355, CRC Press.
Kengen et al., "Purification and characterization of an extremely thermostable .beta.-glucosidase from the hyperthermophilic archaeon Pyrococcus furiosus", Eur. J. Biochem. 213, pp. 305-312 (1993).
Costantino et al., "Purification and characterization of an .alpha.-glucosidase from a hyperthermophilic archaebacterium, Pyrococcus furiosus, exhibiting a temperature optimum of 105 to 115.degree.C", Journal of Bacteriology, 172 (7), pp. 3654-3660, Jul. 1990.
Wozney et al., "Using purified protein to clone its gene", Method Enzymol, 182, pp. 738-749, 1990.
Cubellis et al., "Isolation and sequencing of a new .beta.-galactosidase-encoding archaebacterial gene", Gene 94, pp. 89-94, 1990.
Moracci et al., "Expression of the thermostable .beta.-galactosidase gene from the arcbaebacterium Sulfolobus solfataricus in Saccharomyces cerevisiae and characterization of a new inducible promoter for heterologous expression", Journal of Bacteriology, vol. 174, No. 3, pp. 873-882, Feb. 1992.
Pisani et al., "Thermostable .beta.-galactosidase from the archaebacterium Solfolobus solfataricus purification and properties", Eur. J. Biochem. 187, pp. 321-328, 1990.
M.V. Cubellis et al., "Isolation and Sequencing of a New .beta.-Galactosidase-Encoding Archaebacterial Gene", Gene 94: 89-94, 1990.

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