Hyperimmune composition for passive immunization of fishes again

Details Hyperimmune composition for passive immunization of fishes again Hyperimmune composition for passive immunization of fishes again

1990-07-27

1991-11-12

Page, Thurman K.

Drug, bio-affecting and body treating compositions

Lymphokine

424 92, 424531, 530387, 530388, A61K 3900, A61K 3902, A61K 3514

Patent

active

050646451

ABSTRACT:
The present invention relates to a serum for passive immunization of fish against furunculosis caused by Aeromonas salmonicida, which comprises antibodies against A. salmonicida isolated from fish naturally infected with furunculosis in an intraperitoneal injectable carrier. There is also provided a method for preparing a serum for passive immunization of fish against furunculosis caused by Aeromonas salmonicida, comprising the steps of: a) administering to a mammal selected from the group consisting of bovine, ovine, caprine and equine a sufficient amount of A. salmonicida to produce antibodies; b) collecting from the mammal the serum containing the antibodies against A. salmonicida; c) precipitating and purifying said antibodies by a single precipitation step with an aqueous ammonium sulphate solution in a concentration of about 40% weight/volume and separating the purified precipitated antibodies; d) lyophilizing the purified antibodies; and e) reconstituting the lyophilized antibodies of step d) at about 5 to 7.times.concentration in an intraperitoneal injectable carrier.

REFERENCES:
patent: 4223014 (1980-09-01), Garrison et al.
Ellis et al. Chem. Absts. 109 (11) 90872g.

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