Hyperexpression of bioelastic polypeptides

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Using a micro-organism to make a protein or polypeptide

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435 691, 435 697, 4352523, 536 231, 536 234, 530300, 530350, C12N 120, C12N 121, C12N 1511, C12P 2102

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060047828

ABSTRACT:
A method for overexpressing a bioelastic polypeptide in a host cell is provided. A nucleic acid encoding a bioelastic polypeptide having pentapeptide, tetrapeptide, hexapeptide or nonapeptide repeating units is introduced into a host cell. The host cell is grown under conditions that provide for expression of the polypeptide as at least 40% of the total cellular protein of the host cell.

REFERENCES:
patent: 4132746 (1979-01-01), Urry et al.
patent: 4187852 (1980-02-01), Urry et al.
patent: 4474851 (1984-10-01), Urry
patent: 4500700 (1985-02-01), Urry
patent: 4589882 (1986-05-01), Urry
patent: 4605413 (1986-08-01), Urry et al.
patent: 4693718 (1987-09-01), Urry et al.
patent: 4783523 (1988-11-01), Urry et al.
patent: 4870055 (1989-09-01), Urry et al.
patent: 4898926 (1990-02-01), Urry
patent: 4952496 (1990-08-01), Studier et al.
patent: 4976734 (1990-12-01), Urry et al.
patent: 5032271 (1991-07-01), Urry
patent: 5064430 (1991-11-01), Urry
patent: 5085055 (1992-02-01), Urry
patent: 5226292 (1993-07-01), Urry
patent: 5250516 (1993-10-01), Urry
patent: 5255518 (1993-10-01), Urry
Bitter Methods in Enzymology vol. 152 673-684, 1987.
Zhang et al BioTechnology Letters vol. 17 No. 12 1279-1284, Dec. 1995.
Grosso et al Matrix vol. 13 (2) 157-164, 1993.
Salerno et al Polym Mater Sci Eng vol. 33 p. 398, 1992.
Kuliopulos et al J Am Chem Soc vol. 116 4599-4607, 1994.
Firek et al Plant Molecular Biology vol. 23 861-870, 1993.
Kelley et al Gene vol. 156 33-36.
Mertens et al Bio/Technology vol. 13 175-179, Feb. 1995.
Kane et al Trends In Biotechnology vol. 6 95-101, 1988.
Brosius, (1984) "Plasmid Vectors for the Selection of Promoters" Gene 27:151-160.
Brosius, (1984) "Toxicity of an Overproduced Foreign Gene Product in Escherichia coli and Its Use In Plasmid Vectors for the Selection of Transcription Terminators" Gene 27:161-172.
Daniell et al., (1994) "Engineering Plants for Stress Tolerance via Organelle Genomes" in Biochemical and Cellular Mechanisms of Stress Tolerance in Plants, J.H. Cherry, Ed. (Springer Verlag, NY) pp. 589-604.
Dubendorff and Studier, (1991) "Controlling Basal Expression in an Inducible T7 Expression System by Blocking the Targer T7 Promoter with lac Repressor" J. Mol. Biol 19:45-59.
Gribskov and Burgess, (1983) "Overexpression and Purification of the Sigman Subunit of Escherichia coli RNA Polymerase" Gene 26:109-118.
Hartley and Kane, (1988) "Recovery and Reactivation of Recombinant Proteins" Biochemical Society Transactions 16:101-102.
Kane and Hartley, (1988) "Formation of Recombinant Protein Inclusion Bodies in Escherichia coli" Tibech 6:95-101.
Masiu, et al., (1984) "Novel High-Level Expression Cloning Vehicles: 10.sup.4 -Fold Amplification of Escherichia coli Minor Protein" Biotechnology 2:81-85.
McPherson et al., (1992) "Production and Purification of a Recombinant Elastomeric Polypeptide, G-(VPGVG).sub.19 -VPGV, from Escherichia coli."Biotechnol. Prog. 8:347-352.
Rapaka et al., (1978) "Coacervation Properties in Sequential Polypeptide Models of Elastin" Int. J. Peptide Protein Res. 12:81-92.
Rosenberg et al., (1987) "Vectors for Selective Expression of cloned DNAs by T7 RNA Polymerase" Gene 56:125-135.
Schoner, et al., (1985) "Isolation and Purification of Protein Granules From Escherichia coli Cells Overproducing Bovine Growth Hormone" Biotechnology 3:151-154.
Studier et al., (1990) "Use of T7 RNA Polymerase to Direct Expression of Cloned Genes" Methods in Enzymology 185:60-89.
Studier et al., (1986) "Use of Bacteriophage T7 RNA polymerase to Direct Selective High-level Expression of Cloned Genes" J. Mol. Biol. 189:113-130.
Studier, F.W., (1991) "Use of Bacteriophage T7 Lysozyme to Improve and Inducible T7 Expression System" J. Mol. Biol. 219:37-44.
Tartof and Hobbs, (1987) "Improved Media for Growing Plasmid and Cosmid Clones" Besthesda Res. Lab. Focus 9:12.
Urry et al., (1985) "Carbon-13 NMR Relaxation Studies Demonstrate and Inverse Temperature Transition in the Elastin Polypentapeptide" Biochemistry 24:5182-5188.
Urry et al., (1992) "Hydrophobicity Scale for Proteins Based on Inverse Temperature Transitions" Biopolymers 32:1243-1250.
Urry, (1993) "Molecular Machines: How Motion and Other Functions of Living Organisms Can Result From Reversible Chemical Changes" Angewandte Chemie: A Journal of the Gesellschaft Deutscher Chemiker 32 (6) :819-841.
Urry, et al., (1992) "Design at Nanometric Dimensions To Enhance Hydrophobicity-Induced pK.sub.a Shifts" J. Am. Chem. Soc. 114:8716-8717.
Urry, et al., (1993) "Delineation of Electrostatic- and Hydrophobic-Induced pK.sub.a Shifts in Polypentapeptides: The Glutamic Acid Residue" J. Am. Chem. Soc. 115:7509-7510.
Urry et al., (1991) "Poly (Val.sup.1 -Pro.sup.2 -Ala.sup.3 Val.sup.4 -Gly.sup.5): A Reversible, Inverse Thermoplastic" in Biotechnology and Polymers, C.G. Gebelin, ed. (Plenum Press, NY) pp. 265-274.
Urry et al., (1993) "Medical Applications of Bioelastic Materials" in Biotechnological Polymers: Medical, Pharmaceutical and Industrial Applications, pp. 82-103.
Williams, et al., (1982) "Cytoplasmic Inclusion Bodies in Escherichia coli Producing Biosynthetic Human Insulin Proteins" Science 215:687-689.
Novagen Catalogue (1995) pp. 10, 24-29, 31, 58-62, 78-95.
Urry et al., Protein-Based Polymeric Materials (Synthesis and Properties), pp. 7263-7279, (1996).
Zhang et al., "Nuclear Expression of an Environmentally Friendly Synthetic Protein Based Polymer Gene in Tobacco Cells," Biotechnology Letters, vol. 17(12):1279-1284 (1985).

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