Hyperactive light signal related molecule of...

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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C536S023100, C536S023200

Reexamination Certificate

active

10641100

ABSTRACT:
This invention is about the functionally hyperactive light signal related molecule, HFR1-ΔN105, of which the nucleic acids that encode N-terminal 105 amino acid residues were deleted. HFR1 as a bHLH transcription factor functions in a subset of phytochrome A signaling cascade and it was reported to be regulated negatively by COP1. Experiments with a HFR1-ΔN105 overexpressing plant revealed that the deletion of N-terminal amino acids makes the HFR1 more active in photomorphogenic development such as germination and de-etiolation. In addition, the transgenic plants showed hypersensitive photo-responses in the inhibition of hypocotyl elongation, dependently on another positive element of light signaling, a bZIP protein, HY5. The end-of-day far-red light response and petiole elongation were suppressed in the HFR1-ΔN105 overexpressing plants. These results suggest that N-terminal region of HFR1 negatively regulate HFR1 function and that HFR1-ΔN105 is hyperactive.

REFERENCES:
Thierry Desnos et al.:FHY1: a phytochrome A-specific signal transducer, Genes & Development 15:2980-2990, 2001.
Albrecht G. von Arnim et al.:Light Inactivation of Arabidopsis Photomorphogenic Repressor COP1 Involves a Cell-Specific Regulation of Its Nucleocytoplasmic Partitioning, CELL, vol. 79, 1035-1045, Dec. 16, 1994.
Christian Fankhauser et al.:RSF1, an Arabidopsis Locus Implicated in Phytochrome A Signaling; Plant Physiology, Sep. 2000, vol. 124, pp. 39-45.
Enamul Huq, et al.:PIF4, a phytochrome-interacting bHLH factor, functions as a negative regulator of phytochrome B signaling in Arabidopsis; The EMBO Journal, vol. 21, No. 10, pp. 2441-2450, 2002.
Ference Nagy et al.:Phytochromes Control Photomorphogeneis by Differentially Regulated, Interacting Signaling Pathways in Higher Plants; Annu. Rev. Plant Biol., 53:329-355, 2002.
G. C. Whitelam, et al.: Roles of different phytochromes in Arabidopsis photomorphogeneis; Plant, Cell and Environment, 20:752-758 (1997).
Yoshiharu Y. Yamamoto et al.: CIP4, A New COP1 Target, Is a Nucleus-Localized Positive Regulator of Arabidopsis Photomorphogenesis; The Plant Cell, vol. 13, 399-411, 2001.
Jason W. Reed et al.: Phytochrome A and Phytochrome B Have Overlapping but Distinct Functions in Arabidopis Development; Plant Physiol. 104: 1139-1149, 1994.
Young-Mi Kim et al.;HFR1, a phytochrome A-signalling component, acts in a separate pathway from HY5, downstream of COP1 in Arabidopsis thaliana; The Plant Journal 30(6): 711-719, 2002.
Craig D. Fairchild et al.: HFR1 encodes an atypical bHLH protein that acts in phytochrome A signal transduction; Genes & Development 14:2377-2391, 2000.

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