Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues
Patent
1997-06-18
2000-06-06
Huff, Sheela
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
530324, 424401, 424455, 424451, 514 8, C07K 500
Patent
active
060720301
DESCRIPTION:
BRIEF SUMMARY
TECHNICAL FIELD
The present invention relates to hydroxyproline-rich glycoproteins which can be obtained from vegetable sources, and to the pharmaceutical and cosmetic use thereof.
More precisely, the invention relates to hydroxyproline-rich glycoproteins, which can be obtained by acid alcohol extraction from Taxus spp., Gingko biloba, Lycopersicum esculentum and Daucus carota cell cultures, having the following characteristics: 38,000, determined by means of gel permeation and electrophoresis;
Some glycoproteins of animal origin, such as collagen and proteoglycans, are known to exert a beneficial action on the skin when applied topically as such or incorporated in suitable formulations.
BACKGROUND ART
Collagen, which is a glycoprotein rich in proline and hydroxyproline, is especially used as such or combined with other polypeptide bases in the treatment of wrinkles and other unaesthetic blemishes linked to poor skin hydration and elasticity. The animal origin of collage, however, limits its use because of the risks of contamination from viruses and toxins. Though the compounds of vegetable origin do not involve these risks, so far their use in cosmetics has been quite limited: for examples, cosmetic formulations are known which contain raw extracts of such plants as Aloe or even entire minced vegetables such as avocado.
Vegetable glycoproteins, called extensines, that are produced from vegetable cells in the proliferation stage and have a similar structure to animal collagen, are known. EP-A-0 533 4078 discloses the cosmetic use of extensines having an average molecular weight above 100,000 Daltons. However, the methods for the extraction of extensines described to date, which involve the extraction of vegetable materials of various origin by means of aqueous saline solutions, followed by purification with strong acids such as trichloroacetic acid, do not allow to obtain suitable products for cosmetics, due to problems concerning solubility, stability, repeatability and consistency of their chemical-physical characteristics.
SUMMARY OF THE INVENTION
It has now been found that it is possible to obtain hydroxyproline-rich glycoproteins, structurally similar to the above described extensines but with a lower molecular weight and a higher solubility in acid aqueous solutions, by means of a procedure comprising the in vitro culture of cells of selected plants and the extraction, with acid alcoholic solutions, of the cells grown in a suitable medium.
DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS
The glycoproteins obtainable according to the invention have hydrating, film-forming, toning and cicatrizant properties higher than those of collagen. The glycoproteins of this invention can therefore be employed in cosmetic or dermatologic formulations for the treatment of dry skin, psoriasis, ichtyosis, dandruff, keratosis, wrinkles, acne, eczema, inflammatory dermatosis, ageing of the skin and all the other applications for which the use of animal collagen has been proposed.
The aqueous solutions of the glycoproteins of the invention remain stable without any polymerisation of the glycoproteins leading to the formation of insoluble products. In addition, the viscosity of these solutions is particularly high and not dependent on the concentrations; 0.1% concentrations surprisingly have the same film-forming and hydrating power equal as 1% collagen or 5% vegetable albumin solutions.
The vegetable material to be extracted is obtained from fermenter cultures of Taxus spp., Gingko biloba, Lycopersicum esculentum and Daucus carota cells. The use of cells from the species Taxus spp., Gingko biloba and Lycopersicum esculentum is particularly preferred. The cell culture techniques are conventional and include the suspension culture starting from callus cultures from various parts of the plants such as leaves, bark, roots, trunk or seeds, as described by Dobbs and Roberts, Experiments in Plant Tissue Culture, 2nd ed. Cambridge University Press, New York, 1985.
The vegetable tissue of the callus, following sterili
REFERENCES:
patent: 5443855 (1995-08-01), Wolf et al.
J.P. Stafstrom et al., "A Second Extensin-Like Hydroxyproline-Rich Glycoprotein From Carrot Cell Walls", Plant Physiol., vol. 84 (1987) pp. 820-825.
Schmidt et al Plant. Physiol. vol. 96 p. 656, Nov. 1990.
Chen et al, PNAS, vol. 82 p. 4399, Jul. 1985.
Bombardelli Ezio
Ponzone Cesare
Huff Sheela
Indena S.p.A.
LandOfFree
Hydroxyproline-rich proteins and pharmaceutical and cosmetic for does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Hydroxyproline-rich proteins and pharmaceutical and cosmetic for, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Hydroxyproline-rich proteins and pharmaceutical and cosmetic for will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2214455