Hydrophobic noncovalent binding of proteins to support materials

Details Hydrophobic noncovalent binding of proteins to support materials Hydrophobic noncovalent binding of proteins to support materials

1974-07-29

1977-02-01

Naff, David M.

Textiles: fiber preparation

195 63, 195DIG11, 260112R, C07G 702

Patent

active

040060594

ABSTRACT:
Proteins such as enzymes are noncovalently immobilized by treating a hydrophilic support material having hydroxyl or amino groups with an acid chloride of a hydrophobic acid such as phenoxyacetyl chloride and to the resultant hydrophobic support material adsorbing a protein.

REFERENCES:
patent: 3909360 (1975-09-01), Horiuchi et al.
Er-el, et al., Hydrocarbon-coated Sepharoses, Bichem. and Biophys. Res. Comm., vol. 49 No. 2, 1972, (pp. 383-390).
Yon; R. J. Enzyme Purification by Hydrophobic Chromatography, Biochem. J. vol. 137, Jan. 1974. (pp. 127-130).
Zaborsky; O., Immobilized Enzymes, CRC Press. Cleveland, Ohio, 1973 (pp. 79-82).
Hofstee; B. H. J., Protein Bonding by Agarose Carrying Hydrophobic Groups in Conjunction with Charges, Biochem. and Biophys. Res. Comm. vol. 50, No. 3, 1973 (pp. 751-757).
Hofstee; B. H. J., Hydrophobic Affinity Chromatography of Proteins. Analytical Biochemistry, vol. 52, 1973 (pp. 430-433 and 444-448).

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