Hydrophobic coated membranes

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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Details

435 792, 435 795, 435970, 436518, 436531, 436807, 436810, 427 211, C12Q 168

Patent

active

055276725

ABSTRACT:
A portion of a hydrophobic membrane, having an unknown concentration of a first molecule in a sample bound to its surface, is incubated with a second molecule that selectively binds to the first molecule so as to allow determination of its concentration. The membrane is first coated with the first molecule and then dried to render the uncoated portion nonwettable with water. The labeled second molecule selectively binds to the first molecule in the absence of a blocking agent on the membrane.

REFERENCES:
patent: 4803154 (1989-02-01), Uo et al.
patent: 5200312 (1993-04-01), Oprandy
Sanchez et al., "Improving the detection of proteins after transfer to polyvinylidene difluoride membranes", Electrophoresis, vol. 13, Nos. 9-10, pp. 715-717 (1992).
Pluskal et al., "Immobilon.RTM. PVDF Transfer Membrane: A New Membrane Substrate For Western Blotting of Proteins", vol. 4, No. 3, pp. 272-282 (1986).

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