Liquid purification or separation – With means to add treating material – Chromatography
Reexamination Certificate
2005-02-22
2005-02-22
Therkorn, Ernest G. (Department: 1723)
Liquid purification or separation
With means to add treating material
Chromatography
C210S502100, C210S635000, C210S656000
Reexamination Certificate
active
06858135
ABSTRACT:
Polar, monolithic CEC columns having an amine and/or cyano functional stationary phase are used to separate complex mixtures of saccharides. Using the positive-ion mode of detection for neutral saccharides, detection conditions were optimized to low-femtomole sensitivities with the use of an ion trap mass spectrometer. The described column technology provides a universal system for separating a wide range of carbohydrates: mono- and oligosaccharides with the intact reducing end, as well as saccharide alditols.
REFERENCES:
patent: 5647979 (1997-07-01), Liao et al.
patent: 5935429 (1999-08-01), Liao et al.
patent: 6066258 (2000-05-01), Gjerde et al.
patent: 6372130 (2002-04-01), Gjerde et al.
patent: 20040071968 (2004-04-01), Kyrlidis et al.
Mechref, Y. et al. , “Structural Investigations of Glycocojungates at High Sensitivity”,Chem. Rev., 102: 321-369 (2002).
Mechref, Y. et al., “Mass Spectrometric Mapping and Sequencing of N-Linked Oligosaccharides Derived from Submicrogram Amounts of Glycoproteins”,Anal. Chem., 70:455-463 (1998).
Hong, M. et al., “High-Resolution Studies of Hyaluronic Acid Mixtures through Capillary Gel Electrophoresis”,Anal. Chem., 70:568-573 (1998).
Huang, Y. et al., “Microscale Nonreductive Release of O-Linked Glycans for Subsequent Analysis through MALDI Mass Spectrometry and Capillary Electrophoresis”,Anal. Chem., 73:6063-6069 (2001).
Huang, Y. et al., “Matrix-assisted Laser Desorption/Ionization Mass Spectrometry Compatible β-elimination of O-linked Oligosaccharides”,Rapid Commun. Mass Spectrom., 16:1199-1204 (2002).
Que, A. et al., “Analysis of Bile Acids and Their Conjugates by Capillary Electrochromatography/Electrospray Ion Trap Mass Spectrometry”,Anal.Chem., 72:2703-2710 (2000).
Que, A. et al., “Steroid Profiles Determined by Capillary Electrochromatography, Laser-induced Fluorescence Detection and Electrospray-Mass Spectrometry”,Journal of Chromatography, 887:379-391 (2001).
Que, A. etal., “Separation of Neutral Saccharide Mixtures with Capillary Electrochromatography using Hydrophilic Monolithic Columns”,Anal. Chem., 74:5184-5191 (2002).
Palm, A. et al., “Macroporous Polyacrylamide/Poly(ethylene glycol) Matrixes as Stationary Phases in Capillary Electrochromatography”, Anal. Chem., 69:4499-4507 (1997).
Que et al., “Structural Characterization of Neutral Oligosaccharide Mixtures Through a Combination of Capillary Electrochromatography and Ion Trap Tandem Mass Spectrometry”,Anal. Bioanal. Chem., 375:599-608 (2003).
Que. et al., “Coupling Capillary Electrochromatography with Electrospray Fourier Transform Mass Spectrometry for Characterizing Complex Oligosaccharide Pools”,Anal. Chem., 75:1684-1690 (2003).
Novotny Milos V.
Que Amy H.
Advanced Research and Technology Institute, Inc.
Barnes & Thornburg LLP
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