Hydrophilic monolithic columns for separation of saccharide...

Liquid purification or separation – With means to add treating material – Chromatography

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C210S502100, C210S635000, C210S656000

Reexamination Certificate

active

06858135

ABSTRACT:
Polar, monolithic CEC columns having an amine and/or cyano functional stationary phase are used to separate complex mixtures of saccharides. Using the positive-ion mode of detection for neutral saccharides, detection conditions were optimized to low-femtomole sensitivities with the use of an ion trap mass spectrometer. The described column technology provides a universal system for separating a wide range of carbohydrates: mono- and oligosaccharides with the intact reducing end, as well as saccharide alditols.

REFERENCES:
patent: 5647979 (1997-07-01), Liao et al.
patent: 5935429 (1999-08-01), Liao et al.
patent: 6066258 (2000-05-01), Gjerde et al.
patent: 6372130 (2002-04-01), Gjerde et al.
patent: 20040071968 (2004-04-01), Kyrlidis et al.
Mechref, Y. et al. , “Structural Investigations of Glycocojungates at High Sensitivity”,Chem. Rev., 102: 321-369 (2002).
Mechref, Y. et al., “Mass Spectrometric Mapping and Sequencing of N-Linked Oligosaccharides Derived from Submicrogram Amounts of Glycoproteins”,Anal. Chem., 70:455-463 (1998).
Hong, M. et al., “High-Resolution Studies of Hyaluronic Acid Mixtures through Capillary Gel Electrophoresis”,Anal. Chem., 70:568-573 (1998).
Huang, Y. et al., “Microscale Nonreductive Release of O-Linked Glycans for Subsequent Analysis through MALDI Mass Spectrometry and Capillary Electrophoresis”,Anal. Chem., 73:6063-6069 (2001).
Huang, Y. et al., “Matrix-assisted Laser Desorption/Ionization Mass Spectrometry Compatible β-elimination of O-linked Oligosaccharides”,Rapid Commun. Mass Spectrom., 16:1199-1204 (2002).
Que, A. et al., “Analysis of Bile Acids and Their Conjugates by Capillary Electrochromatography/Electrospray Ion Trap Mass Spectrometry”,Anal.Chem., 72:2703-2710 (2000).
Que, A. et al., “Steroid Profiles Determined by Capillary Electrochromatography, Laser-induced Fluorescence Detection and Electrospray-Mass Spectrometry”,Journal of Chromatography, 887:379-391 (2001).
Que, A. etal., “Separation of Neutral Saccharide Mixtures with Capillary Electrochromatography using Hydrophilic Monolithic Columns”,Anal. Chem., 74:5184-5191 (2002).
Palm, A. et al., “Macroporous Polyacrylamide/Poly(ethylene glycol) Matrixes as Stationary Phases in Capillary Electrochromatography”, Anal. Chem., 69:4499-4507 (1997).
Que et al., “Structural Characterization of Neutral Oligosaccharide Mixtures Through a Combination of Capillary Electrochromatography and Ion Trap Tandem Mass Spectrometry”,Anal. Bioanal. Chem., 375:599-608 (2003).
Que. et al., “Coupling Capillary Electrochromatography with Electrospray Fourier Transform Mass Spectrometry for Characterizing Complex Oligosaccharide Pools”,Anal. Chem., 75:1684-1690 (2003).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Hydrophilic monolithic columns for separation of saccharide... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Hydrophilic monolithic columns for separation of saccharide..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Hydrophilic monolithic columns for separation of saccharide... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3455250

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.