Hydrophilic chemilumescent acridinium labeling reagents

Details Hydrophilic chemilumescent acridinium labeling reagents Hydrophilic chemilumescent acridinium labeling reagents

2005-08-02

2005-08-02

Ceperley, Mary E. (Department: 1641)

Chemistry: analytical and immunological testing

Involving producing or treating antigen or hapten

Producing labeled antigens

C435S006120, C436S500000, C436S526000, C436S544000, C530S391500, C546S102000, C546S104000

Reexamination Certificate

active

06924154

ABSTRACT:
In accordance with the present invention, it has been discovered that introduction of hydrophilic sulfoalkyl substituents and/or hydrophilic linkers derived from homocysteic acid, cysteic acid, glycine peptides, tetraethylene oxide, and the like, offset the hydrophobicity of the acridinium ring system to produce a more soluble label which can be attached to an antibody at higher loading before precipitation and aggregation problems are encountered. Additional compounds described herein contain linkers derived from short peptides and tetraethylene oxide which increase aqueous solubility due to hydrogen bonding with water molecules. The present invention also embraces reagents for multiple acridinium labeling for signal amplification composed of a peptide bearing several acridinium esters with sulfonate groups at regularly spaced intervals for increased solubility. The invention also embraces assays employing the above-described compounds.

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International Search Report for PCT Application No. PCT/US03/25922.

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