Hydrolyzable fluorescent substrates for phosphatases and analyti

Details Hydrolyzable fluorescent substrates for phosphatases and analyti Hydrolyzable fluorescent substrates for phosphatases and analyti

1986-01-31

1989-02-07

Rosen, Sam

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

2605025R, 435 7, 436800, 558210, F12Q 142, C07F 902

Patent

active

048031577

ABSTRACT:
Hydrolyzable substrates for acid and alkaline phosphatases comprise blocked dye moieties which, when cleaved from the substrate during hydrolysis, provide fluorescent dyes having maximum absorptions above about 530 nm and maximum fluorescent emissions at least about 580 nm. The dyes are blocked prior to hydrolysis with a phosphono or thioxophosphono group or a salt thereof. These substrates can be used in analytical determinations of acid or alkaline phosphatase, or in competitive binding reactions to determine immunologically reactive substances.

REFERENCES:
patent: 4051175 (1977-09-01), Andrew et al.
patent: 4279992 (1981-07-01), Boguslaski et al.
patent: 4476229 (1984-10-01), Fino et al.
Koller et al., Amal. Biochem., 143, pp. 146-151 (1984).
Cooke et al., Aust. J. Chem., 11, pp. 230-235 (1958).
Solodar et al., Zhrir. Organ. Khimii, 16 (5), pp. 1062-1064 (1980).

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