Hydrolyzable fluorescent substrates and analytical determination

Details Hydrolyzable fluorescent substrates and analytical determination Hydrolyzable fluorescent substrates and analytical determination

1986-01-31

1989-03-14

Rosen, Sam

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

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Patent

active

048124095

ABSTRACT:
Hydrolyzable substrates comprise blocked dye moieties which, when cleaved from the substrate during hydrolysis, provide fluorescent dyes having maximum absorptions above about 530 nm and maximum emissions at least about 580 nm at physiological pH. These substrates can be used in analytical determinations of hydrolytic substances including hydrolytic enzymes or biological cells containing such enzymes.

REFERENCES:
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patent: 4297273 (1981-10-01), Buckler et al.
patent: 4404366 (1983-09-01), Baguslaski et al.
patent: 4409140 (1983-10-01), Smith et al.
Feng et al, Appl. Env. Microbiol., 43(6), pp. 1320-1329 (1982).
Wasilauskas et al, J. Clin. Microbiol., 20(6) pp. 1205-1206 (1984).
Latt et al, Cytometry, 5, pp. 339-347 (1984).

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