Hydrolysis of protein-bound cholesterol esters

Details Hydrolysis of protein-bound cholesterol esters Hydrolysis of protein-bound cholesterol esters

1979-07-30

1981-06-23

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving cholesterol

435 19, 435198, 435 52, 435822, 435921, 435939, C12Q 160

Patent

active

042751527

ABSTRACT:
A novel process is described for hydrolyzing protein-bound cholesterol esters such as are found in blood serum. The method comprises contacting sample containing protein-bound cholesterol esters with a compatible mixture of an enzyme preparation which demonstrates cholesterol ester hydrolase activity and, as an effector, a surfactant which is an alkyl phenoxy polyethoxy ethanol comprising a polyoxyethylene chain of less than about 20 oxyethylene units.
Hydrolysis compositions comprising compatible mixtures of an enzyme preparation which demonstrates cholesterol ester hydrolase activity and an effector which is a surfactant as described are also disclosed, as are analytical elements comprising at least one layer which includes such a hydrolysis composition.

REFERENCES:
patent: 3689364 (1972-09-01), Hartel
patent: 3703591 (1972-11-01), Bucolo et al.
patent: 3759793 (1973-09-01), Stork
patent: 3869349 (1975-03-01), Goodhue et al.
patent: 3898130 (1975-08-01), Komatsu
patent: 3925164 (1975-12-01), Beaucamp et al.
patent: 4042461 (1977-08-01), Esders et al.
patent: 4052263 (1977-10-01), Masurekov et al.
patent: 4161425 (1979-07-01), Perry
patent: 4164448 (1979-08-01), Roeschlau et al.
Helenius et al., Biochemistry, vol. 10, No. 13, p. 3 (1971).

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