Hydrolysis of protein-bound cholesterol esters

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving cholesterol

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435 19, 435198, 435 52, 435822, 435921, 435939, C12Q 160

Patent

active

042751519

ABSTRACT:
A novel process is described for hydrolyzing protein-bound cholesterol esters such as are found in blood serum. The method comprises contacting sample containing protein-bound cholesterol esters with a compatible mixture of an enzyme preparation which demonstrates cholesterol ester hydrolase activity and, as an effector, a surfactant which is an alkyl phenoxy polyethoxy ethanol comprising a polyoxyethylene chain of less than about 20 oxyethylene units.
Hydrolysis compositions comprising compatible mixtures of an enzyme preparation which demonstrates cholesterol ester hydrolase activity and an effector which is a surfactant as described are also disclosed, as are analytical elements comprising at least one layer which includes such a hydrolysis composition.

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