Hydrolysis of cholinesterase inhibitors using parathion hydrolas

Details Hydrolysis of cholinesterase inhibitors using parathion hydrolas Hydrolysis of cholinesterase inhibitors using parathion hydrolas

1989-02-17

1996-12-31

Naff, David M.

Chemistry: molecular biology and microbiology

Process of utilizing an enzyme or micro-organism to destroy...

Destruction of hazardous or toxic waste

4351723, 435195, 4352523, 43525233, 43525411, 43525421, 435814, 435815, 935 14, 935 29, 935 56, C12S 1300, C12N 1500, C12N 120, C12N 114

Patent

active

055893864

ABSTRACT:
A gene is provided encoding a DNA sequence for the expression of parathion hydrolase and a methionine analog thereof. In a preferred embodiment, a parathion hydrolase gene encoding sequence is obtained in which twenty-eight amino acids are deleted from the N-terminal amino acid sequence of parathion hydrolase. Parathion hydrolase or its analog is produced by transforming a host microorganism such as Escherichia, Bacillus or Streptomyces with the gene, culturing the microorganism in a culture medium and purifying parathion hydrolase therefrom. Highly purified soluble parathion hydrolase is produced by a purification method without using detergents such as Triton X-100 and Tween 20. Parathion hydrolase of enhanced activity is produced by adding cobalt or zinc or a mixture thereof to the culture medium in which the host microorganism is cultured. The parathion hydrolase is useful for hydrolyzing organophosphorus compounds such as the cholinesterase inhibitors, diisopropylphosphoro-fluoridate, pinacolylmethylphosphono-fluoridate and isopropylmethylphosphono-fluoridate.

REFERENCES:
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