Hydrolase purified from human plasma

Details Hydrolase purified from human plasma Hydrolase purified from human plasma

1979-07-02

1980-10-21

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435195, 435212, 435 2, 435 13, 424 94, 424101, C12N 948, C12N 950, C12N 914

Patent

active

042295408

ABSTRACT:
A previously undescribed hydrolytic enzyme has been successfully isolated from human plasma and purified to homogeneity. The enzyme has a molecular weight of about 70,000 daltons, has a Km for the substrate H-D-Phe-Pip-Arg-PNA of about 0.057 mM, a K.sub.I for benzamidine of about 1.1.times.10.sup.-5 M, is bindable to and elutable from insoluble barium salts, is inhibited by antithrombin III, especially in the presence of heparin, and, in vitro, has been found to activate and degrade human Factor VIII, activate human Factor V, and inhibit platelet aggregation induced by ADP, epinephrine, or collagen, thus providing a substance useful for antithrombotic studies.

REFERENCES:
Kisiel et al., Biochemistry, vol. 16, No. 26, pp. 5824-5831 (1977).

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