Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing element or inorganic compound except carbon dioxide
Reexamination Certificate
2006-01-24
2006-01-24
Afremova, Vera (Department: 1651)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing element or inorganic compound except carbon dioxide
C435S257100, C435S257600, C435S946000
Reexamination Certificate
active
06989252
ABSTRACT:
A reversible physiological process provides for the temporal separation of oxygen evolution and hydrogen production in a microorganism, which includes the steps of growing a culture of the microorganism in medium under illuminated conditions to accumulate an endogenous substrate, depleting from the medium a nutrient selected from the group consisting of sulfur, iron, and/or manganese, sealing the culture from atmospheric oxygen, incubating the culture in light whereby a rate of light-induced oxygen production is equal to or less than a rate of respiration, and collecting an evolved gas. The process is particularly useful to accomplish a sustained photobiological hydrogen gas production in cultures of microorganisms, such asChlamydomonas reinhardtii.
REFERENCES:
patent: 4442211 (1984-04-01), Greenbaum
patent: 4532210 (1985-07-01), Miura et al.
Melis, A. et al., “Sustained Photobiological Hydrogen Gas Production Upon Reversible Inactivation of Oxygen Evolution in Green AlgaChlamydomonas reinhardtii,” Proceedings of the 1999 U.S. DOE Hydrogen Program Review, NREL/CP-570-26938.
Benemann, J.R., “Hydrogen Biotechnology Progress and Prospects,” Nature Biotechnol. 14: 1101-1103 (1996).
Benemann John R.
Forestier Marc
Ghirardi Maria
Melis Anastasios
Seibert Michael
Afremova Vera
Midwest Research Institute
White Paul J.
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