Hydrogen bond labeling and base sequence determination methods f

Details Hydrogen bond labeling and base sequence determination methods f Hydrogen bond labeling and base sequence determination methods f

1993-03-04

1995-11-28

Parr, Margaret

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 2532, C12Q 168, C07H 2100

Patent

active

054707072

ABSTRACT:
A hydrogen bond labeling and base sequence determination method for DNA or RNA takes advantage of the complementary nature of the fundamental structure of DNA or RNA bases to bind corresponding chemical species with the base species of a denatured DNA or RNA chain. After providing an aqueous solution containing a nucleic acid, heating the aqueous solution to cleave the nucleic acid; cooling the aqueous solution and adding a base-specific labeling molecule having available hydrogen bonds to the aqueous solution, the base-specific labeling molecules bond to the available bases in a one-to-one fashion via the available hydrogen bonds. The resulting labeled molecule can be microscopically observed to determine the base sequence of the nucleic acid.

REFERENCES:
patent: 5106729 (1992-04-01), Lindsay et al.
S. L. Commerford, Biochemistry, "Iodination of Nucleic Acids in Vitro", vol. 10, No. 11, pp. 1993-1999 (1971).
Matthews, J. A., et al. Review: Analytical Strategies for the Use of DNA Probes, Anal. Biochem (1988) 169:1-25.
UK Patent GB2235049, published Feb. 20, 1991, Scanning microscopes for chemical bond microscopy. (Abstract).

Affiliated with

Also associated with

Rating

Hydrogen bond labeling and base sequence determination methods f does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Hydrogen bond labeling and base sequence determination methods f, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Hydrogen bond labeling and base sequence determination methods f will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2012674