Hybridomas producing monoclonal antibodies to mono-, di- and tri

Details Hybridomas producing monoclonal antibodies to mono-, di- and tri Hybridomas producing monoclonal antibodies to mono-, di- and tri

1989-07-25

1992-12-15

Doll, John J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

5303913, 5303875, 5303881, 5303888, 424 11, 424 9, 424 858, 424 8591, 4351722, 435 7021, 43524027, 935 92, 935104, 935107, C12P 2108, C07K 1528, A61K 4300, C12N 520

Patent

active

051716674

ABSTRACT:
Hybridoma cell lines that produce monoclonal antibodies that differentially recognize glycolipids with mono-, di-, and trifucosylated type 2 chain structures are disclosed. The monoclonal antibodies can be used to detect specific types of tumor cells that are characterized by enrichment in mono-, di-, or trifucosylated type 2 chain structure. As such, the antibodies produced by the hybridoma cell lines are useful for diagnosis and treatment of human cancer. Also disclosed is an improved method of raising hybridoma cell lines by selecting the hybridomas by positive reactively with one or more fucosylated type 2 chain structures selected from the group consisting of III.sup.3 FucnLc.sub.4, V.sup.3 FucnLc.sub.6, III.sup.3 FucnLc.sub.6, III.sup.3 V.sup.3 Fuc.sub.2 nLc.sub.6, and III.sup.3 V.sup.3 VII.sup.3 Fuc.sub.3 nLc.sub.8.

REFERENCES:
patent: 4678747 (1987-07-01), Lloyd et al.
patent: 4876199 (1989-10-01), Hakomori et al.
Fukushi, Y. et al. "Novel Fucolipids accumulating in human adenocarcinoma, II, Selective isolation of hybridoma antibodies that differentially recognize mono-, d-, and trifucosylated type 2 chain", J. Biol. Chem. 259(7):4681-4685, Apr. 10, 1984.
Kannagi, R. et al. "A series of human erythrocyte glycosphinolipids reacting to the monoclonal antibody directed to a developmentally regulated antigen, SSEA-1," J. Biol Chem. 257(24):14865-14874, 1982.
Itzkowitz, S. H., et al., Lewis.sup.x -and sialylated Lewis.sup.x -related antigen expression in human malignant and nonmalignant colonic tissues, Cancer Res. 46:2627-2632, 1986.
Fukushi, Y., et al., Changes of Le.sup.x and dimeric Le.sup.x haptens and their sialylated antigens during development of human kidney and kidney tumors, J. Urol. 135:1048-1056, 1986.
Yuan, M., et al., Expression of Lewis.sup.x and sialylated Lewis.sup.x antigens in human colorectal polyps, JNCI 78(3):479-488, 1987.
Kim, Y. S., et al., Le.sup.x and Le.sup.y antigen expression in human pancreatic cancer, Cancer Res. 48:475-482, 1988.
Ochi, T., et al., The presence of a myeloid cell population showing strong reactivity with monoclonal antibody directed to difucosyl type 2 chain in epiphyseal bone marrow adjacent to joints affected with rheumatoid arthritis (RA) and its absence in the corresponding normal and non-RA bone marrow, J. Rheumatol. 15(11):1609-1615, 1988.
Nakasaki, H., et al., Mosaicism in the expression of tumor-associated carbohydrate antigens in human colonic and gastric cancers, Cancer Res. 49:3662-3669, Jul. 1, 1989.
Anonymous, Center research targets tumors with radiation, Quest 4(2), pp. 1, 4, and 6, Apr. 1990.

Affiliated with

Also associated with

Rating

Hybridomas producing monoclonal antibodies to mono-, di- and tri does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Hybridomas producing monoclonal antibodies to mono-, di- and tri, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Hybridomas producing monoclonal antibodies to mono-, di- and tri will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2092195