Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...
Patent
1985-04-04
1989-10-24
Moskowitz, Margaret
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Blood proteins or globulins, e.g., proteoglycans, platelet...
43524027, 435 68, 4351722, 530809, 935104, 935110, 436548, C12N 500, C07K 1504
Patent
active
048761990
ABSTRACT:
Hybridoma cell lines that produce monoclonal antibodies that differentially recognize glycolipids with mono-, di-, and trifucosylated type 2 chain structures are disclosed. The monoclonal antibodies can be used to detect specific types of tumor cells that are characterized by enrichment in mono-, di-, or trifucosylated type 2 chain structure. As such, the antibodies produced by the hybridoma cell lines are useful for diagnosis and treatment of human cancer. Also disclosed is an improved method of raising hybridoma cell lines by selecting the hybridomas by positive reactivity with one or more fucosylated type 2 chain structures selected from the group consisting of III.sup.3 FucnLc.sub.4, V.sup.3 FucnLc.sub.6, III.sup.3 FucnLc.sub.6, III.sup.3 V.sup.3 Fuc.sub.2 nLc.sub.6, and III.sup.3 V.sup.3 VII.sup.3 Fuc.sub.n nLc.sub.8.
REFERENCES:
Fukuski, Y. "Novel Fucolipids Accumulating in Human Adenocarcinoma II, Selective Isolation of Hybridoma Antibodies That Differentially Recognize Mono-, Di-, and Trifucosylated Type 2 Chain," The Journal of biological Chemistry, vol. 259, No. 7, pp. 4681-4685, 1984.
Abe, K. "The Monoclonal Antibody Directed to Difucosylated Type 2 Chain (Fuc.beta.1.fwdarw.2 Gal.beta.1.fwdarw.4[Fuc.alpha.1.fwdarw.3]GlcNAc; Y Determinant)," The Journal of Biological Chemistry, vol. 258, No. 19, Oct. 10, 1983, pp. 11793-11797, 1983.
The Journal of Biological Chemistry vol. 259 (No. 16):10511-10517, 1984.
J. Exp. Med. vol. 159:506-520, 1984.
The Journal of Biological Chemistry vol. 259 (No. 7):4672-4680, 1984.
Biochemical and Biophysical Research Communications vol. 113 (No. 3):791-798, 1983.
The Journal of Biological Chemistry vol. 257(No. 24):14865-14874, 1982.
Biochemical and Biophysical Research Communications vol. 109 (No. 1):36-44, 1982.
Biochemical and Biophysical Research Communications vol. 100 (No. 4):1578-1586, 1981.
J. Exp. Med. vol. 150:1008-1019, 1979.
The Journal of Biological Chemistry vol. 246 (No. 5):1192-1200, 1971.
Cheney Kay E.
Fred Hutchinson Cancer Research Center
Moskowitz Margaret
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