Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1993-04-13
1996-04-02
Hutzell, Paula K.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
43524027, 4351722, 435 7021, 5303888, 53038885, 5303887, G01N 3353, C07K 1630, C12N 520
Patent
active
055039847
ABSTRACT:
A unique monoclonal antibody against P-glycoprotein. The monoclonal antibody is different than those previously described and has the surprising property of reacting to a soluble form of P-glycoprotein. The invention further includes a novel hybridoma cell line which produces the antibody. A preferred embodiment of the novel antibody has been designated F4 which is believed to react at or near an extracellular transmembrane loop of P-glycoprotein selected from the group consisting of the third and sixth extracellular loops. The invention further comprises a method for detecting the presence of P-glycoprotein comprising reacting a specimen containing P-glycoprotein with the novel monoclonal antibody and detecting the reaction to show that P-glycoprotein is present. The invention, in a preferred embodiment, comprises detecting the presence of drug resistant carcinoma cells, in the absence of biopsy, comprising removing extracellular fluids from a patient for use as a specimen, and determining the presence of P-glycoprotein in the specimen by reacting the specimen with the the novel monoclonal antibody to show that P-glycoprotein is present as an indicator of drug resistance. The extracellular fluid may be any suitable fluid which could contain P-glycoprotein as an indicator of drug resistance. Such fluids may for example be plasma, lymph excretions, and especially ascites taken from the area of a tumor site.
REFERENCES:
patent: 4837306 (1989-06-01), Ling et al.
Juranka et al., "P-glycoprotein: Multidrug-Resistance and a Superfamily of Membrane-Associated Transport Proteins", The FASEB Journal, vol. 3, pp. 2583-2592, (1989).
Bell et al., "Multidrug Resistance: A Novel Class of Membrane-Associated Transport Proteins is Identified", Cancer Investigation, 9(5), 563-569, (1991).
Pastan et al., "Molecular Manipulations of the Multidrug Transporter: A New Role for Transgenic Mice", The FASEB Journal, vol. 5, pp. 2523-2528, (1991).
Roninson et al., "Isolation of Human mdr DNA Sequences Amplified in Multidrug-resistant KB Carcinoma Cells", Proc. Natl. Acad. Sci., vol. 83, pp. 4538-4542, (1986).
Kartner et al., "Detection of P-glycoprotein in Multidrug-resistant Cell Lines by Monoclonal Antibodies", Nature, vol. 316, pp. 820-823, (1985).
Lathan et al., "Immunological Detection of Chinese Hamster Ovary Cells Expressing a Multidrug Resistance Phenotype", Cancer Research 45, pp. 5064-5069, (1985).
Hamada et al., "Functional Role for the 170- to 180-kDa Glycoprotein Specific to Drug-Resistant Tumor Cells as Revealed by Monoclonal Antibodies", Proc. Natl. Acad. Sci., vol. 83, pp. 7785-7789, (1986).
Scheper et al., "Monoclonal Antibody JSB-1 Detects a Highly Conserved Epitope on the P-Glycoprotein Associated with Multi-Drug-Resistance", Int. J. Cancer: 42, pp. 389-394, (1988).
Meyers et al., "Characterization of Monoclonal Antibodies Recognizing a Mr 180,000 P-Glycoprotein: Differential Expression of the Mr 180,000 and Mr 170,000 P-Glycoproteins in Multidrug-resistant Human Tumor Cells", Cancer Research 49, pp. 3209-3214, (1989).
Chabner et al., "Multidrug Resistance: P-glycoprotein and Its Allies--The Elusive Foes", Journal of the National Cancer Institute, vol. 81, No. 12, (1989).
Dalton et al., "Does P-Glycoprotein Predict Response to Chemotherapy, And If So, Is There A Reliable Way to Detect it?", Journal of the National Cancer Institute, vol. 83, No. 2, (1991).
Beck, "Do Anti-P-Glycoprotein Antibodies Have a Future in the Circumvention of Multidrug Resistance?" Journal of the National Cancer Institute, vol. 83, No. 19, pp. 1364-1366, (1991).
Rogan et al., "Reversal of Adriamycin Resistance by Verapamil in Human Ovarian Cancer", Science, vol. 224, pp. 994-996, (1984).
Cowan et al., "Similar Biochemical Changes Associated with Multidrug Resistance in Human Breast Cancer Cells and Carcinogen-induced Resistance to Xenobiotics in Rats", Proc. Natl. Acad. Sci. USA, vol. 83, pp. 9328-9332, (1986).
Akiyama et al., "Isolation and Genetic Characterization of Human KB Cell Lines Resistant to Multiple Drugs", Somatic Cell and Molecular Genetics, vol. 11, No. 2, pp. 117-126, (1985).
Biedler et al., "Drug Resistance in Chinese Hamster Lung and Mouse Tumor Cells", Cancer Treatment Reports, vol. 67, No. 10, pp. 859-867, (1983).
Godling, "Antibody Production by Hybridomas", Journal of Immunological Methods, 39, pp. 285-308, (1980).
Papsidero et al., "Monoclonal Antibodies (F36/22 and M7/105) to Human Breast Carcinoma", Cancer Research 43, pp. 1741-1747, (1983).
Riordan et al., "Purification of P-Glycoprotein from Plasma Membrane Vesicles of Chinese Hamster Ovary Cell Mutants with Reduced Colchicine Permeability", The Journal of Biological Chemistry, vol. 254, No. 24, pp. 12701-12705, (1979).
Peterson, "A Simplification of the Protein Assay Method of Lowry et al. which is More Generally Applicable", Analytical Biochemistry, 83, pp. 346-356, (1977).
Sonoda et al., "Studies of .sup.125 I Trace Labeling of Immunoglobulin G by Chloramine-T", Immunochemistry, vol. 7, pp. 885-898, (1970).
Gerhard et al., "Applications of Monoclonal Antibodies in Virology", In: Weir, D. M. (ed.), Handbook of Experimental Immunology, vol. 4, pp. 115.1-115.13, London: Blackwell Sci. Publ, (1986).
Chu et al., "Prostate-Specific Antigenic Domain of Human Prostate Specific Antigen Identified with Monoclonal Antibodies", The Journal of Urology, vol. 141, pp. 152-156, (1989).
Papsidero et al., "Immunoaffinity Isolation of Ductal Carcinoma Antigen Using Monoclonal Antibody F36/22", Molecular Immunology, vol. 21, No. 10, pp. 955-960, (1984).
Weiss et al., "Effects of Cytoskeletal Perturbation on the Sensitivity of Ehrlich Ascites Tumor Cell Surface Membranes to Mechanical Trauma", Invasion Metastasis, vol. 11, pp. 93-101, (1991).
Towbin et al., "Electrophoretic Transfer of Proteins from Polyacrylamide Gels to Nitrocellulose Sheets: Procedure and Some Applications", Proc. Natl. Acad. Sci., vol. 76, No. 9, pp. 4350-4354, (1979).
Laemmli, "Cleavage of Structural Proteins During the Assembly of the Head of Bacteriophage T4", Nature, vol. 227, pp. 680-685, (1970).
Croghan et al., "Tissue Distribution of an Epithelial and Tumor-associated Antigen Recognized by Monoclonal Antibody F36/22", Cancer Research 43, pp. 4980-4988, (1983).
Willingham et al., "Immunocytochemical Localization of P170 at the Plasma Membrane of Multidrug-resistant Human Cells", The Journal of Histochemistry and Cytochemistry, vol. 35, No. 12, pp. 1451-1456, (1987).
Georges et al., "Detection of P-glycoprotein Isoforms by Gene-specific Monoclonal Antibodies", Proc. Natl. Acad. Sci., vol. 87, pp. 152-156, (1990).
Danks et al., "Monoclonal Antibodies to Glycoproteins of Vinca Alkaloid-resistant Human Leukemic Cells", Cancer Research 45, pp. 3220-3224, (1985).
Chen et al., "Internal Duplication and Homology with Bacterial Transport Proteins in the mdrl (P-Glycoprotein) Gene from Multidrug-Resistant Human Cells", Cell, vol. 47, 381-389, (1986).
Pauletti et al., "Application of a Modified Computer Algorithm in Determining Potential Antigenic Determinants Associated with the AIDS Virus Glycoprotein", Analytical Biochemistry 151, pp. 540-546, (1985).
Thiebaut et al., "Cellular Localization of the Multidrug-resistant gene product P-glycoprotein in Norman Human Tissues", Proc. Natl. Acad. Sci., vol. 84, pp. 7735-7738, (1987).
Georges et al. J. Biol. Chem 268(3):1792-1798 Jan. 25, 1993.
Chu Tsann M.
Kawinski Elzbieta
Lin Tsung-hsing
Dunn Michael L.
Health Research , Inc.
Hutzell Paula K.
LandOfFree
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