Hybridoma producing a monoclonal antibody for N-peptide

Details Hybridoma producing a monoclonal antibody for N-peptide Hybridoma producing a monoclonal antibody for N-peptide

1997-08-08

1998-10-27

Chin, Christopher L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 78, 435 79, 435 792, 435 794, 435 795, 435176, 435960, 436518, 436542, 436547, 436548, 436811, 5303881, G01N 3353, G01N 33543

Patent

active

058276742

ABSTRACT:
The present invention relates to a sandwich immunoassay for rapidly and readily measuring N-peptide using two kinds of monoclonal antibodies recognizing different portions of the N-peptide. The method for measuring N-peptide or a precursor thereof includes the steps of: incubating a mixture containing a sample and a first monoclonal antibody recognizing a portion of N-peptide; adding a labelled second monoclonal antibody recognizing a portion of N-peptide to the mixture, followed by further incubation; and detecting the resulting antigen-antibody complex in the mixture. Alternatively, the method includes the steps of: incubating a mixture containing a sample, a first monoclonal antibody recognizing a portion of N-peptide, and a labelled second monoclonal antibody recognizing another portion of N-peptide; and detecting the resulting antigen-antibody complex.

REFERENCES:
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M.G. Buckley et al., "Concentrations of N-Terminal ProANP in Human Plasma: Evidence for ProANP (1-98) As The Circulating Form," Clinica Chimica Acta, 191, pp. 1-14 (1990).
M.G. Buckley et al., "N-Terminal Pro Atrial Natriuretic Peptide in Human Plasma," American J. Hypertension, 3, pp. 933-935 (1990).
E. Ishikawa et al., Enzyme Immunoassay, 3rd Ed., pp. 75-126 (1987).
H. Itoh et al., ".gamma.-Atrial Natriuretic Polypeptide (.gamma.ANP)-Derived Peptides in Human Plasma: Cosecretion of N-Terminal .gamma.ANP Fragment and .alpha.ANP," J. Clinical Endocrinology and Metabolism, 67, pp. 429-437 (1988).
B.F. Lindberg et al., "Radio-immunoassay of Atrial Natriuretic Peptide (ANP) and Characterization of ANP Immunoreactivity in Human Plasma and Atrial Tissue," Scand. J. Clin. Lab. Invest., 52, pp. 447-456 (1992).
M. Mukoyama et al., "Preparation of Monoclonal Antibodies Against Atrial Natriuretic Polypeptide Precursor and Application to Highly Sensitive Sandwich Enzyme Immunoassay," J. Hypertension, 6 (suppl 4), pp. S320-S322 (1988).
J.A. Sundsfjord et al., "Identification and Plasma Concentrations of the N-Terminal Fragment of Proatrial Natriuretic Factor in Man," J. Clinical Endocrinology and Metabolism, 66, pp. 605-610 (1988).
T. Tsuji et al., "Stability of Human Atrial Natriuretic Peptide in Blood Samples," Clinica Chimica Acta, 225, pp. 171-177 (1994).

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