Hybridoma cell lines producing monoclonal antibodies directed ag

Details Hybridoma cell lines producing monoclonal antibodies directed ag Hybridoma cell lines producing monoclonal antibodies directed ag

1984-07-16

1986-10-21

Hazel, Blondel

Chemistry: molecular biology and microbiology

Spore forming or isolating process

424 85, 530387, 4351722, C12N 500, A61K 39395, C07K 1500

Patent

active

046185852

ABSTRACT:
Continuous hybrid cell lines for producing monoclonal antibodies, the antibodies specific for an antigenic determinant unique to cervical cancer cells, have been developed. The hybrid cell lines were established by fusing differentiated lymphoid cells primed with intact human cervical cancer cells with myeloma cells, particularly plasmacytoma cells. The resulting fused hybrid cells were cultured in HAT tissue culture media which included a small concentration of deoxycytidine. Deoxycytidine was found to enhance the growth of the hybrid cells and subsequent yield of monoclonal antibodies secreted therefrom. Hybrid cell lines secreting monoclonal antibodies to antigenic determinants unique for human cervical cancer cells can be maintained indefinitely in culture to produce large amounts of homogenous anti-cervical cancer cell antibody.

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Schlon et al., "Generation of Human Monoclonal Antibodies Reactive with Human Mammary Carcinoma Cells" Proc. Natl. Acad. Sci. USA 77:6841, (Nov. 1980).
Dippold et al., "Cell Surface Antigens of Human Malignant Melanoma: Definition of Six Antigenic Systems with Mouse Monoclonal Antibodies" Proc. Natl. Acad. Sc: USA 77:6114 (Oct. 1980).
Glass, David, "Medicine's Micro Missiles" Science Digest, pp. 30, 31, 114, (Jan. 1982).
Lakachura et al., "Monoclonal Antibodies Against Human Hela Cell Surface Antigens" Abstract of Tissue Culture Association (Mar. 20, 1981).
Wiels et al., "Monoclonal Antibody Against a Burkitt Lymphoma Associated Antigen", Proc. Natl. Acad. Sci. USA 78:6485, (Oct. 1981).

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