Hybridoma antibody which inhibits interleukin 2 activity

Details Hybridoma antibody which inhibits interleukin 2 activity Hybridoma antibody which inhibits interleukin 2 activity

1981-10-07

1984-09-25

Wiseman, Thomas

Chemistry of carbon compounds

Miscellaneous organic carbon compounds

C-metal

4351722, 435240, 435241, 424 85, 436548, C07G 700, C12N 1500, C12N 500, C12N 502, A61K 3900

Patent

active

044734930

ABSTRACT:
A process for producing anti-IL-2 antibody from hybridoma cells generated by fusing activated, IL-2 immunized, murine lymphocyte cells with neoplastic murine myeloma cells. Fusion is accomplished by mixing the two cell lines together in the presence of a fusing agent. After fusion, the hybridoma cells are cultured in vitro in a supplemented tissue culture medium to thereby produce anti-IL-2 antibody. Also, the hybridoma cells are cloned by a limiting dilution procedure to isolate even more potent sources of anti-IL-2 antibody. Anti-IL-2 antibody is then purified from either tissue culture medium conditioned by hybridoma cells, or from peritoneal ascites of mice challenged with hybridoma cells.

REFERENCES:
Stadler et al: Fed. Proc. 40(3 part II), 1111, Abstr. 5002, (1981).
Gillis et al., "Long Term Culture of Tumour-Specific Cytotoxic T Cells", 268, Nature, 154, (1977).
Gillis et al., "T Cell Growth Factor: Parameters of Production and a Quantitative Microassay for Activity", 120 J. Immunol. 2027, (1978).
Watson et al., "Biochemical and Biological Characterization of Lymphohocyte Regulatory Molecules-I. Purification of a Class of Murine Lymphokines", 150, J. Exp. Med., 849, (1979).
Gillis et al., "Biochemical Characterization of Lymphocyte Regulatory Molecules-II. Purification of a Class of Rat and Human Lymphokines", 124, J. Immunol. 154, (1980).
Luben et al., "Production of Hybridomas Secreting Monoclonal Antibodies Against the Lymphokine Osteoclast Activity Factor", 154 Journal of Clinical Investigation, 1262, (1979).
Luben et al., "Use of In Vito Immunization in Production of Monoclonal Antibodies Against Osteoclast-Activating Factor: A Method of General Applicability to Lymphokines", Biochemical Characterization of Lymphokines, edited by DeWeck et al., Academic Press, N.Y., p. 321, (1979).

Affiliated with

Also associated with

Rating

Hybridoma antibody which inhibits interleukin 2 activity does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Hybridoma antibody which inhibits interleukin 2 activity, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Hybridoma antibody which inhibits interleukin 2 activity will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1768871