Hybridoma antibody which inhibits interleukin 2 activity

Details Hybridoma antibody which inhibits interleukin 2 activity Hybridoma antibody which inhibits interleukin 2 activity

1981-04-29

1983-10-25

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435240, 435241, 435317, 435 41, 435 70, 435948, 424 85, C12P 2100, C12N 500, C12N 502, C12N 100, C12P 100, C12P 2102, C12R 191, A61K 3900

Patent

active

044119933

ABSTRACT:
A process for producing anti-IL-2 antibody from hybridoma cells generated by fusing activated, IL-2 immunized, murine lymphocyte cells with neoplastic murine myeloma cells. Fusion is accomplished by mixing the two cell lines together in the presence of a fusing agent. After fusion, the hybridoma cells are cultured in vitro in a supplemented tissue culture medium to thereby produce anti-IL-2 antibody. Also, the hybridoma cells are cloned by a limiting dilution procedure to isolate even more potent sources of anti-IL-2 antibody. Anti-IL-2 antibody is then purified from either tissue culture medium conditioned by hybridoma cells, or from peritoneal ascites of mice challenged with hybridoma cells.

REFERENCES:
patent: 4196265 (1980-04-01), Koprowski et al.
Harwell et al.; J. Exp. Med. 152, 893 (1980).

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