Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2005-07-19
2005-07-19
Horlick, Kenneth R. (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S007100
Reexamination Certificate
active
06919180
ABSTRACT:
The invention relates to kits and methods for hybridizing nucleic acids with a specific nucleic acid-binding agent, such as a complementary nucleic acid. Previously, others have hybridized a nucleic acid with such an agent bound to a substrate. The improved methods described herein comprise binding a polycationizable attractor compound to the substrate, in addition to the agent. Examples of suitable polycationizable attractor compounds include polypeptides, including those with tunable cationizable amino acid residues, such as histidine. Compositions, kits, devices, and methods that make use of this hybridization rate enhancement technology are disclosed.
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Belosludtsev, Y., et al., “Nearly Instantaneous, Cation-Independent, High Selectivity Nucleic Acid Hybridization to DNA Microarrays”, Biochem Biophys Rsch Comm, 2001, vol. 282(5), pp. 1263-1267.
Zhang, P., et al., “Acceleration of Nucleic Acid Hybridization on DNA Microarrays Driven by pH Tunable Modifications”, Nucleosides, Nucleotides & Nucleic Acids, 2001, vol. 282(4-7), pp. 1251-1254.
Gunning Kerry B.
Hogan Michael
Powdrill Tom
Horlick Kenneth R.
Senniger Powers
Sigma Genosys, L.P.
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