Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1994-08-11
1996-12-31
Zitomer, Stephanie W.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
935 78, 935 79, 435967, C12Q 168
Patent
active
055893350
ABSTRACT:
Novel reagents are provided along with methods for their use in nucleic acid hybridization assays for detecting target nucleic acids in samples. The most preferred reagent is a mixture of guanidinium thiocyanate and tetramethylammonium:Trifluoracetate and possesses many properties for facilitating hybridization between target nucleic acid and nucleic acid probes capable of binding thereto and surprisingly results in the phenomenon of superstoichiometric labeling.
REFERENCES:
Thompson et al., Anal. Biochem. 163(2):281-291, 1987.
Orosy, DNA Iodination, Provirus Isolation, Excluded Volume and Electrostatic Effects on DNA Renaturation Rate, Dissertation Information Service, 1975, pp. 1-4 and 167-176.
Melchior et al., Proc. Natl Acad Sci. 70(2):298-02, 1973.
Douglass et al., Methods of Enzymology 124:309-318, 1986.
Hames et al, Nucleic Acid Hybridization, IRL Press, Wash., D.C., 1985, p. 6.
Rawn, Biochemistry, Harper & Row, N.Y., N.Y. 1983, p. 1055.
Wood et al., Proc. Natl.Acad.Sci. 82:1585-88, 1985.
DiLella et al, Method in Enzymology 152:447-451.
Devlin et al., DNA 7(7):499-05, 1988.
Collins Mark L.
Eldredge John K.
Kearney Kevin R.
Morrissey David V.
Amoco Corporation
Galloway Norval B.
Houtteman Scott W.
Zitomer Stephanie W.
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