Hybridization method of detecting nucleic acid sequences with pr

Details Hybridization method of detecting nucleic acid sequences with pr Hybridization method of detecting nucleic acid sequences with pr

1984-06-01

1987-03-03

Marantz, Sidney

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 78, 436501, 536 27, C12Q 168, C12P 1956, G01N 33566, C07H 1512

Patent

active

046475290

ABSTRACT:
Improved hybridization probe compositions and method for detecting nucleic acids are provided. A hybridization probe constructed to contain thionucleotides is hybridized to a nucleotide sequence of interest in the absence of formamide, DTT and dextran sulfate. If a cold (.sup.32 S) thionucleotide is incorporated into the probe molecule, the probe is provided with a detectable label, radioactive or otherwise.

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