Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1986-12-19
1990-03-13
Yarbrough, Amelia Burgess
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
436501, 536 27, 935 1, 935 7, 935 16, 935 23, 935 78, 935 79, 935 88, C12Q 168, G01N 33566, C07H 1512, C12N 1500
Patent
active
049083074
ABSTRACT:
Improved hybridization probe compositions and method for detecting nucleic acids of interest are provided. A hybridization probe constructed to contain thionucleotides and a detectable label is hybridized to a nucleotide sequence of interest in the absence of nonpolar solvents, reducing agents and volume exclusion agents. The thionucleotides serve to increase the mass of bound probe molecules, thereby amplifying the signal available to detect the presence of the nucleic acid sequence of interest. Certain cations interact specifically with the thiol moiety of phosphorothioate groups of nucelic acid molecules in a manner that can be exploited to amplify probe binding or convey a detectable label. Thus, the mass of bound probe molecules can be further increased by the presence of specified multivalent cations from the transition metal group, such as Cu.sup.2+ or Fe.sup.3+, during the annealing step.
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Pecoraro, Hermes and Cleland,
This invention was made in part with Government support, and the Government has certain rights in the invention.
Rodland Karin D.
Russell Peter J.
Burgess Yarbrough Amelia
Marschel Ardin
Rodland Karin D.
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